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National Institute of Environmental Health Sciences

 

Person Details

Superfund Research Program

Sylvia Daunert

University of Miami
University of Miami
1011 NW 15 St.
Gautier Bldg, Room 116
Miami, FL 33136
Phone: 305-243-4005
Fax: 305-243-3955
Email:

Projects

Research Briefs

Patents

  • Spores for the Stabilization and On-Site Application of Bacterial Whole-Cell Biosensing Systems

Publications

2013
  • Choi, Jeong-June, Sung Yong Eum, Evadnie Rampersaud, Sylvia Daunert, Maria T. Abreu, and Michal Toborek. 2013. Exercise attenuates PCB-induced changes in the mouse gut microbiome. Environmental Health Perspectives. 121(6):725-730. doi:10.1289/ehp.1306534  
  • Zhang, Bei, Jeong-June Choi, Sung Yong Eum, Sylvia Daunert, and Michal Toborek. 2013. TLR4 signaling is invovled in brain vascular toxicity of PCB153 bound to nanoparticles. PLoS ONE.   8(5):e63159. doi:10.1371/journal.pone.0063159  
2012
2011
  • Hamorsky, K.T., E. Dikici, C.M. Ensor, and Sylvia Daunert. 2011. Designer Bioluminescent Systems in Biotechnology. Chapter 13 in: Chemiluminescence and Bioluminescence: Past, Present and Future. Royal Society of Chemistry, Cambridge, United Kingdom.
  • Knecht, Leslie D., Patrizia Pasini, and Sylvia Daunert. 2011. Bacterial spores as platforms for bioanalytical and biomedical applications. Analytical and Bioanalytical Chemistry.   400(4):977-989. doi:10.1007/s00216-011-4835-4  
  • Lewis, Scott, Saurav Datta, Minghui Gui, Eric Coker, Frank Huggins, Sylvia Daunert, Leonidas G. Bachas, and Dibakar Bhattacharyya. 2011. Reactive nanostructured membranes for water purification. Proceedings of the National Academy of Sciences of the United States of America (PNAS).   108(21):8577-8582. doi:10.1073/pnas.1101144108  
  • Sangal, Abhishek, Patrizia Pasini, and Sylvia Daunert. 2011. Stability of spore-based biosensing systems under extreme conditions. Sensors and Actuators B: Chemical.   158:377-382. doi:10.1016/j.snb.2011.06.039  
  • Turner, K., N. Raut, Patrizia Pasini, Sylvia Daunert, E. Michelini, L. Cevenini, L. Mezzanotte, and A. Roda. 2011. Cell-Based Bioluminescent Biosensors. Chapter 15 in: Chemiluminescence and Bioluminescence: Past, Present and Future. Royal Society of Chemistry, Cambridge, United Kingdom. pp.514-545.
2010
  • Date, A., Patrizia Pasini, and Sylvia Daunert. 2010. Fluorescent and bioluminescent cell-based sensors: strategies for their preservation. Advances in Biochemical Engineering/Biotechnology.   117:57-75.
  • Date, A., Patrizia Pasini, and Sylvia Daunert. 2010. Integration of spore-based genetically engineered whole-cell sensing systems into portable centrifugal microfluidic platforms. Analytical and Bioanalytical Chemistry.   398(1):349-356. doi:10.1007/s00216-010-3930-2  
  • Date, A., Patrizia Pasini, A. Sangal, and Sylvia Daunert. 2010. Packaging Sensing Cells in Spores for Long-Term Preservation of Sensors: A Tool for Biomedical and Environmental Analysis. Analytical Chemistry.   82(14):6098-6103. doi:10.1021/ac1007865  
  • Struss, Anjali, Patrizia Pasini, and Sylvia Daunert. 2010. Biosensing Systems Based on Genetically Engineered Whole Cells. Chapter 14 in: Recognition Receptors in Biosensors. Springer, New York, NY. pp.565-598.
2009
2006
  • Daunert, Sylvia. 2006. Analytical and bioanalytical chemistry in white waters: 2006 and beyond. Analytical and Bioanalytical Chemistry.   384(1):1-3.
  • Feliciano, Jessika S., Yue Liu, and Sylvia Daunert. 2006. Novel reporter gene in a fluorescent-based whole cell sensing system. Biotechnology and Bioengineering.   93(5):989-997.
  • Feliciano, Jessika S., Patrizia Pasini, Sapna K. Deo, and Sylvia Daunert. 2006. Photoproteins as reporters in whole cell sensing systems. Chapter 8 in: Photoproteins in Bioanalysis. Wiley-VCH, New York, NY. pp.131-154.
  • Feliciano, Jessika S., Shifen Xu, Xiyuan Guan, Hans-Joachim Lehmler, Leonidas G. Bachas, and Sylvia Daunert. 2006. ClcR-based biosensing system in the detection of cis-dihydroxylated (chloro-)biphenyls. Analytical and Bioanalytical Chemistry.   385(5):807-813.
2005
  • Rothert, Anna, Sapna K. Deo, Lori Millner, Libby G. Puckett, Marc J. Madou, and Sylvia Daunert. 2005. Whole-cell-reporter-gene-based biosensing systems on a compact disk microfluidics platform. Analytical Biochemistry.   342(1):11-19.
  • Xu, Shifen, Elisa D'Angelo, Dhritiman Ghosh, Jessika S. Feliciano, Sapna K. Deo, and Sylvia Daunert. 2005. Detection of polychlorinated biphenyls employing chemical dechlorination followed by biphenyl whole cell sensing system. Toxicological & Environmental Chemistry.   87(3):287-298. doi:10.1080/02772240400029769  
2003
  • Feliciano, Jessika S., Xiyuan Guan, Youchin Liu, Elisa D'Angelo, Jan Roelof van der Meer, Leonidas G. Bachas, and Sylvia Daunert. 2003. Bacterial sensing systems for monitoring superfund chemicals. In: Proceedings of the NAFF (North American Freedom Foundation) Symposium, 2003. Lexington, KY.
  • Feliciano, Jessika S., Xiyuan Guan, Jan Roelof van der Meer, Leonidas G. Bachas, and Sylvia Daunert. 2003. Bacterial Biosensing Systems for Monitoring EnvironmentalPollutants:From The Laboratory to the Field. In: Proceedings of the International Symposium on Sensor Science, 2003. Paris, France.
  • Feliciano, Jessika S., Anna Rothert, Sapna K. Deo, Libby G. Puckett, Lori Millner, Jan Roelof van der Meer, Marc J. Madou, and Sylvia Daunert. 2003. Bacterial biosensing systems for arsenic detection: from the laboratory to the field. In: Proceedings of the Superfund Basic Research Program Annual Meeting: Integration Perspectives, November 9-12, 2003. Dartmouth College, Hanover, NH.
  • Rothert, S., Sapna K. Deo, Libby G. Puckett, Lori Millner, Marc J. Madou, and Sylvia Daunert. 2003. Adaptation of a whole-cell based reporter gene assay for arsenite and antimonite to a compact disc centrifugal microfluidics patform. In: Proceedings of the 55th Southeast Regional Meeting of the American Chemical Society (SERMACS), November 16-19, 2003. Atlanta, GA.
  • Stocker, Judith, Denisa Balluch, Monika Gsell, Hauke Harms, Jessika S. Feliciano, Sylvia Daunert, Khurseed A. Malik, and Jan Roelof van der Meer. 2003. Development of a set of simple bacterial biosensors for quantitative and rapid measurements of arsenite and arsenate in potable water. Environmental Science & Technology.   37(20):4743-4750.
  • Xu, S., Amlan Ghosh, Jessika S. Feliciano, Sapna K. Deo, Elisa D'Angelo, and Sylvia Daunert. 2003. Development of a whole-cell sensing system for PCBs detection. In: Proceedings of the Superfund Basic Research Program Annual Meeting: Integration Perspectives, November 9-12, 2003. Dartmouth College, Hanover, NH.
2002
2001
  • Butterfield, D.Allan, Dibakar Bhattacharyya, Sylvia Daunert, and Leonidas G. Bachas. 2001. Catalytic biofunctional membranes containing site-specifically immobilized enzyme arrays: a review. Journal of Membrane Science.   181(1):29-37.
  • Ramanathan, Sridhar, Ranjit S. Shetty, and Sylvia Daunert. 2001. Genetically Engineered Microorganisms as Biosensors for Environmental Toxins. In: Proceedings of the 9th European Congress on Biotechnology.
2000
  • Daunert, Sylvia, Gary Barrett, Jessika S. Feliciano, Ranjit S. Shetty, Suresh Shrestha, and Wendy Smith-Spencer. 2000. Genetically engineered whole-cell sensing systems: coupling biological recognition with reporter genes. Chemical Reviews.   100(7):2705-2738.
  • Guan, Xiyuan, Sridhar Ramanathan, J.P. Garris, Ranjit S. Shetty, C. Mark Ensor, Leonidas G. Bachas, and Sylvia Daunert. 2000. Chlorocatechol detection based on a clc operon/reporter gene system. Analytical Chemistry.   72(11):2423-2427.
1999
  • Ramanathan, Sridhar, Youchin Liu, Ranjit S. Shetty, Xiyuan Guan, and Sylvia Daunert. 1999. Luminescence-Based Sensing Systems Employing Genetically Engineered Bacteria. In: Proceedings of the 10th International Symposium on Bioluminescence and Chemiluminescence. John Wiley & Sons, Chichester, England. pp.601-604.
1998
  • Bachas, Leonidas G., Sylvia Daunert, and Larry W. Robertson. 1998. Information dissemination and outreach activities at the University of Kentucky. In: Proceedings of the Superfund Basic Research Program (SBRP) Conference, 1998. Boston, MA. pp.69.
  • Hernandez, Emily C. and Sylvia Daunert. 1998. Green Fluorescent Protein as a Novel Label in Binding Assays. Analytical Biochemistry.   261(1):113-115.
  • Lewis, Jennifer C., Agatha Feltus, C. Mark Ensor, Sridhar Ramanathan, and Sylvia Daunert. 1998. Applications of Reporter Genes in Analytical Chemistry. Analytical Chemistry.   70:579A-585A.
  • Ramanathan, Sridhar, Jennifer C. Lewis, Mark S. Kindy, and Sylvia Daunert. 1998. Heterogeneous Binding Assay for an Octapeptide Based on a Fusion Protein with Recombinant Aequorin. Analytica Chimica Acta.   369:181-188.
  • Ramanathan, Sridhar, Weiping Shi, Barry P. Rosen, and Sylvia Daunert. 1998. Bacteria-Based Chemiluminescence Sensing System Using ±-Galactosidase Under the Control of the ArsR Regulatory Protein of the ars Operon. Analytica Chimica Acta.   369:189-195.
  • Salins, Lyndon L., Vesna Schauer-Vukasinovic, and Sylvia Daunert. 1998. Optical Sensing Systems Based on Biomolecular Recognition of Recombinant Proteins. SPIE Proceedings, San Jose, CA. 3270:16-24.
1997
  • Blair, T.L., Sylvia Daunert, and Leonidas G. Bachas. 1997. Host-Guest Chemistryin Fiber Optic Sensing. In: Biosensors, Current Topics in Biophysics.
  • Crofcheck, Czarena L., Anne L. Grosvenor, Kimberly W. Anderson, Janet K. Lumpp, Donna L. Scott, and Sylvia Daunert. 1997. Detecting Biomolecules in pL-Vials Using the Bioluminescence of Aequorin. Analytical Chemistry.   69(23):4768-4772.
  • Ensor, C.Mark, Sridhar Ramanathan, Donna L. Scott, and Sylvia Daunert. 1997. Engineered Bacteria Can Detect Toxic Metals. Waterworks. 3:1-4.
  • Feltus, Agatha, Sridhar Ramanathan, and Sylvia Daunert. 1997. Cooperative Interaction of Immobilized Avidin with Aequorin-Biotin Conjugates: An Aequorin-Linked Assay for Biotin. Analytical Biochemistry.   254:62-68.
  • Grosvenor, Anne L., Czarena L. Crofcheck, Kimberly W. Anderson, Donna L. Scott, and Sylvia Daunert. 1997. Calibration of Micropipets Using the Bioluminescent Protein Aequorin. Analytical Chemistry.   69:3115-3118.
  • Lizano, Sergio, Sridhar Ramanathan, Agatha Feltus, Allan Witkowski, and Sylvia Daunert. 1997. Bioluminescence Competitive Binding Assays for Biotin Based on the Photoprotein Aequorin. Methods in Enzymology.   279:296-303.
  • Palet, Cristina, Maria Muñoz, Manuel Valiente, Tadeusz Cynkowski, Sylvia Daunert, and Leonidas G. Bachas. 1997. Molecular Recognition of Dicarboxylic Acids by Neutral Receptors: Selective Membrane Transport. Analytica Chimica Acta.   343(3):287-294.
  • Ramanathan, Sridhar, C. Mark Ensor, and Sylvia Daunert. 1997. Metal-Resistance Bacterial Systems in Sensors. Trends in Biotechnology.   15(12):500-506.
  • Ramanathan, Sridhar, Weiping Shi, Barry P. Rosen, and Sylvia Daunert. 1997. Bioluminescence Sensing System For Antimonite with Subattomole Detection Based on Luciferase Reporter Genes. Analytical Chemistry.   69:3380-3384.
  • Schauer-Vukasinovic, Vesna, Lawrence Cullen, and Sylvia Daunert. 1997. Rational Design of a Calcium Sensing System Based on Induced Conformational Changes of Calmodulin. Journal of the American Chemical Society.   119:11102-11103.
  • Scott, Donna L., Sridhar Ramanathan, Weiping Shi, Barry P. Rosen, and Sylvia Daunert. 1997. Electrochemical-Based Sensing System for Antimonite and Arsenite Using Genetically Engineered Bacteria. Analytical Chemistry.   69:16-20.
1996
  • Hentz, Nathaniel G. and Sylvia Daunert. 1996. Bifunctional Fusion Proteins of Calmodulin and Protein A as Affinity Ligands in Protein Purification and in the Study of Protein-Protein Interactions. Analytical Chemistry.   68(22):3939-3944.
  • Hentz, Nathaniel G., Vesna Vukasinovic, and Sylvia Daunert. 1996. Affinity Chromatography for Recombinant Peptides/Proteins Based on a Calmodulin Fusion Tail. Analytical Chemistry.   68(9):1550-1555.
  • Hentz, Nathaniel G., Vesna Vukasinovic, Sergi Diez, Manuel Valiente, and Sylvia Daunert. 1996. Affinity Chromatography for Recombinant Proteins. In: Biofunctional Membranes (The Language of Science)   . Plenum Press, pp.73-81.
  • Huang, Wei, Agatha Feltus, Allan Witkowski, and Sylvia Daunert. 1996. Homogeneous Bioluminescence Competitive Binding Assay for Folate Based on a Coupled Glucose-6-phosphate Dehydrogenase-Bacterial Luciferase System. Analytical Chemistry.   68(9):1646-1650.
1995
  • Allen, John R., Antonio Florido, Shelley D. Young, Sylvia Daunert, and Leonidas G. Bachas. 1995. Nitrite-Selective Electrode Based on an Electropolymerized Cobalt Phthalocyanine. Electroanalysis.   7(8):710-713.
  • Daunert, Sylvia, Leonidas G. Bachas, and T. Smith-Palmer. 1995. Gas Sensing Probes. In: Encyclopedia of Analytical Science. Academic Press, pp.4118-4124.
  • Diez, Sergi, Pu Zhou, Sylvia Daunert, and Manuel Valiente. 1995. Immobilized Soft-Metal Affinity System for Amino Acids Based on an 8-Hydroxyquinoline-Pd(II) Complex: Characterization Using Glycine as a Model. Analytica Chimica Acta.   315:339-345.
  • Hernandez, Emily C., Allan Witkowski, Sylvia Daunert, and Leonidas G. Bachas. 1995. Potentiometric Enzyme Electrodes for Urea Based on Electrochemically Prepared Polypyrrole Membranes. Microchimica Acta.   121:63-72.
  • Witkowski, Allan, Sylvia Daunert, Mark S. Kindy, and Leonidas G. Bachas. 1995. Preparation of β-Galactosidase Conjugates for Competitive Binding Assays by Post-Translational Modification of Recombinant Proteins. Analytical Chemistry.   67:1301-1306.