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Study Information
Study Attribute Value
CEBS Accession Number: 002-01536-0001-0000-8
Chemical Name: 5-Azacytidine
CASRN: 320-67-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 932806_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 07/19/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1039 427 0.411 8.54 26.5 0.000
Test Chemical 5-Azacytidine 
 0.16      
 50 1033 594 0.575 11.88 26.5 39.918 
 0.5       
 50 1041 631 0.606 12.62 26.5 47.491 
1.6       
 50 1043 688 0.660 13.76 26.5 60.506 
5         
 50 1042 710 0.681 14.20 26.5 65.798 
16         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1046 489 0.467 9.78 26.5 13.754
Mitomycin-C 
 0.001     
 10 210 136 0.648 13.60 26.5 57.582
Trend: 8.330        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 04/02/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 419 0.400 8.38 26.0 0.000
Test Chemical 5-Azacytidine 
 0.16      
 50 1047 469 0.448 9.38 26.5 11.933 
 0.5       
 50 1055 517 0.490 10.34 26.5 22.453 
1.6       
 50 1050 536 0.510 10.72 26.5 27.558 
5         
 20 421 254 0.603 12.70 31.0 50.759 
10         
 0 0 0 0.000 0.00 31.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1050 561 0.534 11.22 26.0 33.508
Mitomycin-C 
 0.005     
 10 210 250 1.190 25.00 26.0 197.477
Trend: 5.483        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/19/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 412 0.394 8.24 26.0 0.000
Test Chemical 5-Azacytidine 
 0.5       
 50 1049 447 0.426 8.94 26.0 8.185 
1.6       
 50 1040 468 0.450 9.36 26.0 14.248 
5         
 50 1040 503 0.484 10.06 26.0 22.792 
16         
 50 1041 670 0.644 13.40 26.0 63.402 
50         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Cyclophosphamide 
 0.3       
 50 1047 564 0.539 11.28 26.0 36.762
Cyclophosphamide 
1         
 10 208 177 0.851 17.70 26.0 116.045
Trend: 8.220        
Probability: 0.000