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Study Information
Study Attribute Value
CEBS Accession Number: 002-01814-0001-0000-7
Chemical Name: C.I. Vat brown 3
CASRN: 131-92-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 728480_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 06/28/1988    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1040 412 0.396 8.24 26.0 0.000
Test Chemical C.I. Vat brown 3 
16         
 50 1034 458 0.443 9.16 26.0 11.810 
50         
 50 1029 477 0.464 9.54 26.0 17.014 
160         
 50 1045 552 0.528 11.04 26.0 33.340 
500         
 50 1029 817 0.794 16.34 26.0 100.421
Positive Control Mitomycin-C 
 0.0E-4    
 50 1042 560 0.537 11.20 26.0 35.661
Mitomycin-C 
 0.005     
 10 207 322 1.556 32.20 26.0 292.665
Trend: 12.341        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 08/09/1988    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1045 407 0.389 8.14 26.0 0.000
Test Chemical C.I. Vat brown 3 
16         
 50 1042 420 0.403 8.40 26.0 3.491 
50         
 50 1042 399 0.383 7.98 26.0 -1.683 
160         
 50 1038 526 0.507 10.52 26.0 30.110 
500         
 50 1038 828 0.798 16.56 26.0 104.812
Positive Control Mitomycin-C 
 0.0E-4    
 50 1048 493 0.470 9.86 26.0 20.783
Mitomycin-C 
 0.005     
 10 209 258 1.234 25.80 26.0 216.953
Trend: 13.323        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 06/28/1988    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1048 452 0.431 9.04 26.0 0.000
Test Chemical C.I. Vat brown 3 
160         
 50 1043 440 0.422 8.80 26.0 -2.188 
500         
 50 1038 468 0.451 9.36 26.0 4.537 
1600         
 50 1040 545 0.524 10.90 26.0 21.503 
2500         
 50 1038 550 0.530 11.00 26.0 22.854
Positive Control Cyclophosphamide 
 0.15      
 50 1046 581 0.555 11.62 26.0 28.786
Cyclophosphamide 
 0.6       
 10 210 196 0.933 19.60 26.0 116.401
Trend: 4.503        
Probability: 0.000