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Study Information
Study Attribute Value
CEBS Accession Number: 002-02042-0002-0000-2
Chemical Name: Diethylstilbestrol
CASRN: 56-53-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 293260_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Positive 
Induced Rat Liver S9  2_S9  Positive 
Induced Rat Liver S9  3_S9  Positive 

Trial #:1   Activation: No Activation    Date: 01/06/1983    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Diethylstilbestrol 
5         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
7.5       
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
10         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
15         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
20         
 100 4 0.040 4.0 3 0.030 3.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 35 0.350 32.0 19 0.190 19.0 16 0.160 14.0 0 0.000 0.0
Trend: 1.508 1.419 0.614  
Probability: 0.066 0.078 0.270  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 02/06/1983    Harvest Time: 12.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Negative (Not Specified) 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Diethylstilbestrol 
 0.5       
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
2.5       
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
5         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 
7.5       
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
10         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 100 63 0.630 41.0 35 0.350 25.0 23 0.230 19.0 5 0.050 5.0
Trend: 1.813 1.918 0.731  
Probability: 0.035 0.028 0.233  


Trial #:2   Activation: No Activation    Date: 01/27/1983    Harvest Time: 12 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Negative (Not Specified) 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Diethylstilbestrol 
1         
 100 4 0.040 4.0 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 
2.5       
 100 3 0.030 2.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 
5         
 100 8 0.080 7.0 6 0.060 6.0 2 0.020 2.0 0 0.000 0.0 
7.5       
 100 8 0.080 6.0 6 0.060 5.0 2 0.020 2.0 0 0.000 0.0 
10         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
15         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.5       
 100 62 0.620 37.0 36 0.360 30.0 25 0.250 20.0 1 0.010 1.0
Trend: 0.546 0.737 0.493  
Probability: 0.293 0.230 0.311  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 06/19/1983    Harvest Time: 18 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Negative (Not Specified) 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Diethylstilbestrol 
2.5       
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
5         
 100 4 0.040 4.0 3 0.030 3.0 1 0.010 1.0 0 0.000 0.0 
5         
 100 2 0.020 2.0 2 0.020 1.0 0 0.000 0.0 0 0.000 0.0 
7.5       
 100 30 0.300 18.0 13 0.130 9.0 15 0.150 12.0 2 0.020 2.0 
7.5       
 100 148 1.480 49.0 86 0.860 42.0 22 0.220 18.0 40 0.400 4.0 
10         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
10         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 100 39 0.390 30.0 23 0.230 20.0 16 0.160 15.0 0 0.000 0.0
Trend: 9.071 7.801 5.916  
Probability: 0.000 0.000 0.000  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 01/27/1983    Harvest Time: 12 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Diethylstilbestrol 
 0.5       
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
1         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
2.5       
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
5         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
7.5       
 100 85 0.850 77.0 2 0.020 2.0 75 0.750 67.0 8 0.080 8.0 
10         
 100 53 0.530 48.0 4 0.040 4.0 40 0.400 37.0 9 0.090 9.0
Positive Control: Cyclophosphamide 
25         
 100 47 0.470 31.0 15 0.150 11.0 29 0.290 23.0 3 0.030 3.0
Trend: 13.495 2.272 12.129  
Probability: 0.000 0.012 0.000