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Study Information
Study Attribute Value
CEBS Accession Number: 002-03018-0001-0000-5
Chemical Name: Ziram
CASRN: 137-30-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 054163_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Questionable 
Induced Rat Liver S9  2_S9  Negative 
Induced Rat Liver S9  3_S9  Negative 
Induced Rat Liver S9  4_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 05/14/1985    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1051 419 0.399 8.38 26.5 0.000
Test Chemical Ziram 
 0.001     
 50 1049 488 0.465 9.76 26.5 16.690 
 0.005     
 50 1042 480 0.461 9.60 26.5 15.548 
 0.01      
 50 1043 496 0.476 9.92 26.5 19.285 
 0.025     
 50 1038 519 0.500 10.38 26.5 25.418 
 0.05      
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1048 603 0.575 12.06 26.5 44.326
Mitomycin-C 
 0.005     
 10 210 260 1.238 26.00 26.5 210.558
Trend: 3.153        
Probability: 0.001        

Trial #: 2    Activation: No Activation    Date: 08/20/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 473 0.450 9.46 26.5 0.000
Test Chemical Ziram 
 0.001     
 50 1048 500 0.477 10.00 26.5 5.910 
 0.005     
 50 1046 446 0.426 8.92 26.5 -5.348 
 0.01      
 50 1043 505 0.484 10.10 31.5 7.482 
 0.025     
 0 0 0 0.000 0.00 31.5 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1050 623 0.593 12.46 26.5 31.712
Mitomycin-C 
 0.005     
 10 210 243 1.157 24.30 26.5 156.871
Trend: 0.235        
Probability: 0.407        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 05/14/1985    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 415 0.395 8.30 26.0 0.000
Test Chemical Ziram 
 0.5       
 50 1047 387 0.370 7.74 26.0 -6.480 
 0.75      
 50 1046 417 0.399 8.34 26.0 0.866 
1         
 50 1047 405 0.387 8.10 26.0 -2.130 
1.25      
 50 1039 525 0.505 10.50 26.0 27.845
Positive Control Cyclophosphamide 
 0.2       
 50 1049 775 0.739 15.50 26.0 86.925
Cyclophosphamide 
 0.6       
 10 210 283 1.348 28.30 26.0 240.964
Trend: 3.489        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 07/02/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 474 0.453 9.48 26.0 0.000
Test Chemical Ziram 
1.25      
 50 1049 531 0.506 10.62 31.0 11.705 
1.5       
 50 1045 481 0.460 9.62 31.0 1.574 
1.75      
 50 1048 554 0.529 11.08 31.0 16.655 
2         
 0 0 0 0.000 0.00 31.0 N/A
Positive Control Cyclophosphamide 
 0.2       
 50 1048 1060 1.011 21.20 26.0 123.202
Cyclophosphamide 
 0.6       
 10 210 361 1.719 36.10 26.0 279.351
Trend: 1.798        
Probability: 0.036        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 07/23/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 488 0.467 9.76 26.0 0.000
Test Chemical Ziram 
 0.25      
 50 1049 482 0.459 9.64 26.0 -1.512 
 0.5       
 50 1047 504 0.481 10.08 26.0 3.180 
 0.75      
 50 1050 469 0.447 9.38 26.0 -4.260 
1         
 50 1048 523 0.499 10.46 26.0 6.968 
1.25      
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Cyclophosphamide 
 0.2       
 50 1047 1599 1.527 31.98 26.0 227.351
Cyclophosphamide 
 0.6       
 10 211 662 3.137 66.20 26.0 572.492
Trend: 0.700        
Probability: 0.242        

Trial #: 4_S9    Activation: Induced Rat Liver S9    Date: 04/16/1986    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 413 0.394 8.26 26.5 0.000
Test Chemical Ziram 
 0.16      
 50 1048 441 0.421 8.82 26.5 6.780 
 0.5       
 50 1051 491 0.467 9.82 26.5 18.547
Positive Control Cyclophosphamide 
 0.3       
 50 1048 1082 1.032 21.64 26.5 161.985
Cyclophosphamide 
 0.6       
 10 210 341 1.624 34.10 26.5 312.047
Trend: 2.561        
Probability: 0.005