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Study Information
Study Attribute Value
CEBS Accession Number: 002-02235-0010-0000-5
Chemical Name: Hamamelis water (witch hazel)
CASRN: 68916-39-2
NTP Study Type: Genetic Toxicology - Rodent Cytogenetics
NTP Study ID: 042552_CA
Study Result: Negative


Table Instructions and Notes:

Below are the detailed Chromosome Abberation data. You may also view the summary data for this study.


Initial Treatment Date Tissue Sample Time (h) Sex Species Strain Protocol Result
05/04/1987 Bone Marrow 17 Male Mouse B6C3F1 Intraperitoneal Injection x 1/study, 17 hour Negative

  Dose
(mg/kg)		 Animal
Number		 No.
Cells		 Chromatid Isochromatid Exchanges Aberrations
Breaks Gaps Breaks Gaps 'Tid 'Some Other Total Per Cell % Cells w/ Abs
(+ Gaps) (- Gaps) (+ Gaps) (- Gaps)
Positive Control Mitomycin-C 
1         
 5382 50 14 4 6 0 0 0 0 24 0.48 0.40 26.00 24.00
    5383 50 11 12 1 0 0 0 0 24 0.48 0.24 26.00 20.00
    5384 50 1 1 0 0 0 0 0 2 0.04 0.02 2.00 2.00
    5385 50 2 9 0 0 0 0 0 11 0.22 0.04 10.00 2.00
    5386 50 4 5 0 0 0 0 0 9 0.18 0.08 16.00 8.00
    5387 50 4 4 2 0 0 0 0 10 0.20 0.12 14.00 8.00
    5388 50 9 9 8 1 0 0 0 27 0.54 0.34 30.00 26.00
    5390 50 51 4 0 0 0 0 0 55 1.10 1.02 12.00 8.00
Average         12.0 6.0 2.1 0.1 0.0 0.0 0.0 20.3 0.41 0.28 17.00 12.25
± SEM                         0.12 0.12 3.38 3.41
 
Test Chemical Hamamelis water (witch hazel) 
1250         
 5312 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    5313 50 1 0 0 0 0 0 0 1 0.02 0.02 0.00 2.00
    5314 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    5315 50 0 2 0 0 0 0 0 2 0.04 0.00 4.00 0.00
    5316 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    5317 50 1 2 0 0 0 0 0 3 0.06 0.02 6.00 2.00
    5318 50 6 4 0 1 0 0 0 11 0.22 0.12 12.00 10.00
    5319 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
Average         1.0 1.3 0.0 0.1 0.0 0.0 0.0 2.4 0.05 0.02 3.25 1.75
± SEM                         0.03 0.01 1.46 1.22
 
Test Chemical Hamamelis water (witch hazel) 
2500         
 5322 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    5323 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    5324 50 1 0 1 0 0 0 0 2 0.04 0.04 4.00 4.00
    5325 50 4 2 1 0 0 0 0 7 0.14 0.10 10.00 8.00
    5326 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    5327 50 2 1 0 0 0 0 0 3 0.06 0.04 4.00 4.00
    5328 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    5329 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
Average         1.3 0.5 0.3 0.0 0.0 0.0 0.0 2.0 0.04 0.03 3.25 2.75
± SEM                         0.02 0.01 1.13 1.00
 
Test Chemical Hamamelis water (witch hazel) 
5000         
 5332 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    5333 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    5334 50 3 1 0 0 0 0 0 4 0.08 0.06 6.00 6.00
    5335 50 45 6 0 0 0 0 0 51 1.02 0.90 18.00 10.00
    5336 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    5337 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    5338 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    5339 50 2 1 0 0 0 0 0 3 0.06 0.04 6.00 4.00
Average         6.6 1.4 0.0 0.0 0.0 0.0 0.0 8.0 0.16 0.13 5.25 3.25
± SEM                         0.12 0.11 1.96 1.19
 
Vehicle Control No Stressor 
 0         
 5372 50 3 1 0 0 0 0 0 4 0.08 0.06 4.00 2.00
    5373 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    5374 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    5375 50 9 3 0 1 0 0 0 13 0.26 0.18 14.00 12.00
    5376 50 5 7 0 1 0 0 0 13 0.26 0.10 18.00 10.00
    5377 50 5 5 1 0 0 0 0 11 0.22 0.12 14.00 8.00
    5378 50 2 6 1 0 0 0 0 9 0.18 0.06 10.00 6.00
    5379 50 1 0 1 0 0 0 0 2 0.04 0.04 4.00 4.00
Average         3.5 2.8 0.4 0.3 0.0 0.0 0.0 6.9 0.14 0.08 8.75 6.00
± SEM                         0.04 0.02 2.14 1.31
 
Footnotes:
CRO: Corn Oil
DMBA: Dimethylbenzanthracene
IP/IJ: Intraperitoneal injection
INHAL: Inhalation