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Study Information
Study Attribute Value
CEBS Accession Number: 002-02235-0010-0000-5
Chemical Name: Hamamelis water (witch hazel)
CASRN: 68916-39-2
NTP Study Type: Genetic Toxicology - Rodent Cytogenetics
NTP Study ID: 042552_CA
Study Result: Negative


Table Instructions and Notes:

Below are the detailed Chromosome Abberation data. You may also view the summary data for this study.


Initial Treatment Date Tissue Sample Time (h) Sex Species Strain Protocol Result
07/28/1988 Bone Marrow 17 Male Mouse B6C3F1 Intraperitoneal Injection x 1/study, 17 hour Negative

  Dose
(mg/kg)		 Animal
Number		 No.
Cells		 Chromatid Isochromatid Exchanges Aberrations
Breaks Gaps Breaks Gaps 'Tid 'Some Other Total Per Cell % Cells w/ Abs
(+ Gaps) (- Gaps) (+ Gaps) (- Gaps)
Positive Control Mitomycin-C 
1         
 8508 50 5 0 0 0 0 0 0 5 0.10 0.10 8.00 8.00
    8509 50 6 0 1 0 0 0 0 7 0.14 0.14 14.00 14.00
    8510 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    8511 50 2 4 0 0 0 0 0 6 0.12 0.04 10.00 2.00
    8512 50 6 0 0 0 0 0 0 6 0.12 0.12 12.00 12.00
    8513 50 8 2 0 0 0 0 0 10 0.20 0.16 14.00 12.00
    8514 50 5 2 0 0 1 0 0 8 0.16 0.12 14.00 10.00
    8516 50 8 3 1 0 0 0 0 12 0.24 0.18 20.00 14.00
Average         5.0 1.4 0.3 0.0 0.1 0.0 0.0 6.8 0.14 0.11 11.50 9.00
± SEM                         0.03 0.02 2.06 1.89
 
Test Chemical Hamamelis water (witch hazel) 
1250         
 8438 50 4 0 0 0 0 0 0 4 0.08 0.08 8.00 8.00
    8439 50 3 3 1 0 0 0 0 7 0.14 0.08 10.00 8.00
    8440 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    8441 50 0 3 0 0 0 0 0 3 0.06 0.00 4.00 0.00
    8442 50 1 2 0 0 0 0 0 3 0.06 0.02 6.00 2.00
    8443 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    8444 50 7 1 0 0 0 0 0 8 0.16 0.14 12.00 10.00
    8445 50 0 2 0 0 0 0 0 2 0.04 0.00 2.00 0.00
Average         2.3 1.4 0.1 0.0 0.0 0.0 0.0 3.8 0.08 0.05 6.00 4.25
± SEM                         0.02 0.02 1.31 1.39
 
Test Chemical Hamamelis water (witch hazel) 
2500         
 8448 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    8449 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    8450 50 0 0 1 0 0 0 0 1 0.02 0.02 2.00 2.00
    8451 50 1 1 1 0 0 0 0 3 0.06 0.04 6.00 4.00
    8452 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    8453 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    8454 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    8455 50 3 0 0 0 0 0 0 3 0.06 0.06 6.00 6.00
Average         0.8 0.1 0.3 0.0 0.0 0.0 0.0 1.1 0.02 0.02 2.25 2.00
± SEM                         0.01 0.01 0.88 0.76
 
Test Chemical Hamamelis water (witch hazel) 
5000         
 8458 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    8459 50 2 1 0 0 0 0 0 3 0.06 0.04 6.00 4.00
    8460 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    8461 50 1 2 0 0 0 0 0 3 0.06 0.02 6.00 2.00
    8462 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    8463 50 4 0 0 0 0 0 0 4 0.08 0.08 8.00 8.00
    8464 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    8465 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
Average         1.4 0.4 0.0 0.0 0.0 0.0 0.0 1.8 0.04 0.03 3.50 2.75
± SEM                         0.01 0.01 0.98 0.84
 
Vehicle Control No Stressor 
 0         
 8498 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    8499 50 0 3 0 0 0 0 0 3 0.06 0.00 6.00 0.00
    8500 50 3 0 0 0 0 0 0 3 0.06 0.06 6.00 6.00
    8501 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    8502 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    8503 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    8504 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    8505 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
Average         0.6 0.5 0.0 0.0 0.0 0.0 0.0 1.1 0.02 0.01 2.25 1.25
± SEM                         0.01 0.01 0.88 0.75
 
Footnotes:
CRO: Corn Oil
DMBA: Dimethylbenzanthracene
IP/IJ: Intraperitoneal injection
INHAL: Inhalation