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Study Information
Study Attribute Value
CEBS Accession Number: 002-01254-0017-0000-2
Chemical Name: 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride)
CASRN: 15481-70-6
NTP Study Type: Genetic Toxicology - Rodent Cytogenetics
NTP Study ID: 051468_CA
Study Result: Negative


Table Instructions and Notes:

Below are the detailed Chromosome Abberation data. You may also view the summary data for this study.


Initial Treatment Date Tissue Sample Time (h) Sex Species Strain Protocol Result
04/17/1986 Bone Marrow 17 Male Mouse B6C3F1 Intraperitoneal Injection x 1/study, 17 hour Negative

  Dose
(mg/kg)		 Animal
Number		 No.
Cells		 Chromatid Isochromatid Exchanges Aberrations
Breaks Gaps Breaks Gaps 'Tid 'Some Other Total Per Cell % Cells w/ Abs
(+ Gaps) (- Gaps) (+ Gaps) (- Gaps)
Positive Control Mitomycin-C 
1         
 3387 50 4 1 0 0 0 0 0 5 0.10 0.08 10.00 8.00
    3388 50 6 0 0 0 0 0 0 6 0.12 0.12 10.00 10.00
    3393 50 3 2 0 0 0 0 0 5 0.10 0.06 8.00 6.00
    3400 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    3407 50 9 6 0 0 0 0 0 15 0.30 0.18 18.00 16.00
    3408 50 8 4 0 0 0 0 0 12 0.24 0.16 20.00 14.00
    3422 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    3431 50 2 1 0 0 0 0 0 3 0.06 0.04 6.00 4.00
Average         4.4 1.8 0.0 0.0 0.0 0.0 0.0 6.1 0.12 0.09 9.75 8.00
± SEM                         0.03 0.02 2.25 1.77
 
Test Chemical 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) 
75         
 3391 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3394 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3397 50 0 2 0 0 0 0 0 2 0.04 0.00 4.00 0.00
    3406 50 2 1 0 0 0 0 0 3 0.06 0.04 6.00 4.00
    3416 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3420 50 4 2 0 0 0 0 0 6 0.12 0.08 6.00 6.00
    3424 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    3429 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
Average         0.9 0.8 0.0 0.0 0.0 0.0 0.0 1.6 0.03 0.02 2.50 1.50
± SEM                         0.01 0.01 0.91 0.82
 
Test Chemical 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) 
150         
 3389 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3390 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3396 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    3404 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    3414 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    3417 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    3427 50 1 0 0 0 0 0 1 2 0.04 0.04 4.00 4.00
    3430 50 2 1 0 0 0 0 0 3 0.06 0.04 6.00 4.00
Average         0.8 0.5 0.0 0.0 0.0 0.0 0.1 1.4 0.03 0.02 2.75 1.75
± SEM                         0.01 0.01 0.75 0.59
 
Test Chemical 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) 
300         
 3392 50 1 2 0 0 0 0 0 3 0.06 0.02 4.00 2.00
    3402 50 1 1 0 1 0 0 0 3 0.06 0.02 6.00 2.00
    3409 50 4 0 0 0 0 0 0 4 0.08 0.08 8.00 8.00
    3412 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3415 50 0 2 0 0 0 0 0 2 0.04 0.00 4.00 0.00
    3423 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    3425 50 0 2 0 0 0 0 0 2 0.04 0.00 4.00 0.00
    3433 50 1 2 0 0 0 0 0 3 0.06 0.02 6.00 2.00
Average         1.0 1.3 0.0 0.1 0.0 0.0 0.0 2.4 0.05 0.02 4.50 2.00
± SEM                         0.01 0.01 0.82 0.93
 
Vehicle Control No Stressor 
 0         
 3395 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    3399 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    3401 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    3410 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    3411 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    3413 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    3426 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    3428 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
Average         0.6 0.5 0.0 0.0 0.0 0.0 0.0 1.1 0.02 0.01 2.25 1.25
± SEM                         0.00 0.00 0.45 0.37
 
Footnotes:
CRO: Corn Oil
DMBA: Dimethylbenzanthracene
IP/IJ: Intraperitoneal injection
INHAL: Inhalation