Genetic Toxicology - Rodent Cytogenetics

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01265-0007-0000-3
Chemical Name:	2-Acetylaminofluorene
CASRN:	53-96-3
NTP Study Type:	Genetic Toxicology - Rodent Cytogenetics
NTP Study ID:	242744_CA
Study Result:	Positive (Nonstandard Protocol)

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Table Instructions and Notes:

Below are the detailed Chromosome Abberation data. You may also view the summary data for this study.

Initial Treatment Date	Tissue	Sample Time (h)	Sex	Species	Strain	Protocol	Result
05/04/1988	Bone Marrow	36	Male	Mouse	B6C3F1	Intraperitoneal Injection x 1/study, 36 hour	Positive (Nonstandard Protocol)

		Dose (mg/kg)	Animal Number	No. Cells	Chromatid		Isochromatid		Exchanges			Aberrations
					Breaks	Gaps	Breaks	Gaps	'Tid	'Some	Other	Total	Per Cell		% Cells w/ Abs
					Breaks	Gaps	Breaks	Gaps	'Tid	'Some	Other	Total	(+ Gaps)	(- Gaps)	(+ Gaps)	(- Gaps)
Positive Control	Dimethylbenzanthracene	100	7934	50	18	20	1	1	0	0	0	40	0.80	0.38	28.00	18.00
			7935	50	22	6	0	0	0	0	0	28	0.56	0.44	22.00	14.00
			7936	50	101	27	3	2	0	0	0	133	2.66	2.08	32.00	30.00
			7937	50	24	20	5	0	0	0	0	49	0.98	0.58	38.00	24.00
			7938	50	90	19	1	0	0	0	0	110	2.20	1.82	38.00	28.00
			7939	50	113	60	0	2	2	0	0	177	3.54	2.30	64.00	50.00
			7940	50	26	14	0	2	0	0	0	42	0.84	0.52	24.00	18.00
			7941	50	94	24	2	7	0	0	1	128	2.56	1.94	56.00	44.00
Average					61.0	23.8	1.5	1.8	0.3	0.0	0.1	88.4	1.77	1.26	37.75	28.25
± SEM													0.39	0.30	5.32	4.54

Test Chemical	2-Acetylaminofluorene	125	7894	50	0	2	0	0	0	0	0	2	0.04	0.00	2.00	0.00
			7895	50	1	1	0	0	0	0	0	2	0.04	0.02	4.00	2.00
			7896	50	0	4	0	0	0	0	0	4	0.08	0.00	6.00	0.00
			7897	50	1	1	0	0	0	0	0	2	0.04	0.02	4.00	2.00
			7898	50	0	5	0	0	0	0	0	5	0.10	0.00	8.00	0.00
			7899	50	0	0	0	0	1	0	0	1	0.02	0.02	2.00	2.00
			7900	50	3	2	0	0	0	0	0	5	0.10	0.06	8.00	6.00
			7901	50	0	0	0	0	0	0	0	0	0.00	0.00	0.00	0.00
Average					0.6	1.9	0.0	0.0	0.1	0.0	0.0	2.6	0.05	0.02	4.25	1.50
± SEM													0.01	0.01	1.03	0.73

Test Chemical	2-Acetylaminofluorene	250	7904	50	1	9	0	0	0	0	0	10	0.20	0.02	12.00	2.00
			7905	50	2	1	0	0	0	0	0	3	0.06	0.04	4.00	4.00
			7906	50	2	0	0	0	0	0	0	2	0.04	0.04	4.00	4.00
			7907	50	3	2	0	0	0	0	0	5	0.10	0.06	10.00	6.00
			7908	50	3	1	0	0	0	0	0	4	0.08	0.06	8.00	6.00
			7909	50	3	6	0	1	0	0	0	10	0.20	0.06	14.00	6.00
			7910	50	0	0	0	0	0	0	0	0	0.00	0.00	0.00	0.00
			7911	50	0	0	0	0	0	0	0	0	0.00	0.00	0.00	0.00
Average					1.8	2.4	0.0	0.1	0.0	0.0	0.0	4.3	0.09	0.04	6.50	3.50
± SEM													0.03	0.01	1.88	0.91

Test Chemical	2-Acetylaminofluorene	500	7914	50	5	18	0	5	0	0	0	28	0.56	0.10	30.00	16.00
			7915	50	0	5	0	0	0	0	0	5	0.10	0.00	10.00	0.00
			7916	50	0	1	0	1	0	0	0	2	0.04	0.00	4.00	0.00
			7917	50	2	2	0	0	0	0	0	4	0.08	0.04	6.00	4.00
			7918	50	4	2	1	0	0	0	0	7	0.14	0.10	14.00	10.00
			7919	50	1	3	0	0	0	0	0	4	0.08	0.02	8.00	2.00
			7920	50	5	10	1	0	0	0	0	16	0.32	0.12	20.00	12.00
			7921	50	3	8	0	0	0	0	0	11	0.22	0.06	14.00	6.00
Average					2.5	6.1	0.3	0.8	0.0	0.0	0.0	9.6	0.19	0.06	13.25	6.25
± SEM													0.06	0.02	3.00	2.09

Vehicle Control	No Stressor	0	7924	50	0	0	0	0	0	0	0	0	0.00	0.00	0.00	0.00
			7925	50	2	2	0	0	0	0	0	4	0.08	0.04	6.00	2.00
			7926	50	0	3	0	0	0	0	0	3	0.06	0.00	6.00	0.00
			7927	50	2	1	0	0	0	0	0	3	0.06	0.04	4.00	2.00
			7928	50	0	6	0	0	0	0	0	6	0.12	0.00	8.00	0.00
			7929	50	1	3	0	0	0	0	0	4	0.08	0.02	6.00	2.00
			7930	50	0	3	1	0	0	0	0	4	0.08	0.02	6.00	2.00
			7931	50	0	1	0	0	0	0	0	1	0.02	0.00	2.00	0.00
Average					0.6	2.4	0.1	0.0	0.0	0.0	0.0	3.1	0.06	0.02	4.75	1.00
± SEM													0.01	0.01	0.92	0.38

Footnotes:
CRO: Corn Oil
DMBA: Dimethylbenzanthracene
IP/IJ: Intraperitoneal injection
INHAL: Inhalation