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Study Information
Study Attribute Value
CEBS Accession Number: 002-01525-0014-0000-0
Chemical Name: 5,5-Diphenylhydantoin (phenytoin)
CASRN: 57-41-0
NTP Study Type: Genetic Toxicology - Rodent Cytogenetics
NTP Study ID: 252423_CA
Study Result: Negative


Table Instructions and Notes:

Below are the detailed Chromosome Abberation data. You may also view the summary data for this study.


Initial Treatment Date Tissue Sample Time (h) Sex Species Strain Protocol Result
07/29/1987 Bone Marrow 36 Male Mouse B6C3F1 Intraperitoneal Injection x 1/study, 36 hour Negative

  Dose
(mg/kg)		 Animal
Number		 No.
Cells		 Chromatid Isochromatid Exchanges Aberrations
Breaks Gaps Breaks Gaps 'Tid 'Some Other Total Per Cell % Cells w/ Abs
(+ Gaps) (- Gaps) (+ Gaps) (- Gaps)
Positive Control Dimethylbenzanthracene 
100         
 5824 50 21 2 1 0 0 0 0 24 0.48 0.44 20.00 18.00
    5828 50 57 8 0 0 0 0 0 65 1.30 1.14 38.00 32.00
    5831 50 4 7 0 0 0 0 0 11 0.22 0.08 18.00 8.00
    5834 50 46 7 0 0 0 0 0 53 1.06 0.92 30.00 24.00
    5840 50 46 13 1 0 0 0 0 60 1.20 0.94 42.00 30.00
    5849 50 26 7 0 0 0 0 0 33 0.66 0.52 22.00 14.00
    5860 50 50 10 1 1 0 0 1 63 1.26 1.04 42.00 36.00
    5876 50 84 9 0 0 0 0 0 93 1.86 1.68 48.00 38.00
Average         41.8 7.9 0.4 0.1 0.0 0.0 0.1 50.3 1.01 0.85 32.50 25.00
± SEM                         0.19 0.17 4.08 3.84
 
Test Chemical 5,5-Diphenylhydantoin (phenytoin) 
37.5       
 5848 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    5855 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    5859 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    5861 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    5874 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    5879 50 1 0 0 0 0 0 1 2 0.04 0.04 2.00 2.00
    5883 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    5884 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
Average         0.1 0.4 0.0 0.0 0.0 0.0 0.1 0.6 0.01 0.01 1.00 0.25
± SEM                         0.01 0.01 0.38 0.25
 
Test Chemical 5,5-Diphenylhydantoin (phenytoin) 
75         
 5851 50 4 4 1 0 0 0 0 9 0.18 0.10 18.00 10.00
    5852 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    5856 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    5858 50 1 1 0 0 0 0 1 3 0.06 0.04 4.00 2.00
    5867 50 17 2 0 0 0 0 0 19 0.38 0.34 18.00 14.00
    5869 50 0 3 0 0 0 0 1 4 0.08 0.02 6.00 0.00
    5878 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    5881 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
Average         2.9 1.3 0.1 0.0 0.0 0.0 0.3 4.5 0.09 0.07 6.00 3.50
± SEM                         0.05 0.04 2.73 1.92
 
Test Chemical 5,5-Diphenylhydantoin (phenytoin) 
150         
 5850 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    5853 50 0 0 0 0 0 0 1 1 0.02 0.02 2.00 2.00
    5862 50 0 3 0 0 0 0 0 3 0.06 0.00 4.00 0.00
    5864 50 0 0 1 0 0 0 0 1 0.02 0.02 2.00 2.00
    5866 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    5868 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    5875 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    5877 50 3 0 0 0 0 0 0 3 0.06 0.06 6.00 6.00
Average         0.6 0.8 0.1 0.0 0.0 0.0 0.1 1.6 0.03 0.02 3.00 1.75
± SEM                         0.01 0.01 0.65 0.70
 
Vehicle Control No Stressor 
 0         
 5812 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    5818 50 0 2 0 0 0 0 0 2 0.04 0.00 4.00 0.00
    5820 50 3 1 0 0 0 0 0 4 0.08 0.06 8.00 6.00
    5835 50 3 1 0 0 0 0 0 4 0.08 0.06 8.00 6.00
    5854 50 3 2 1 0 0 0 0 6 0.12 0.08 8.00 6.00
    5865 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    5870 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    5885 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
Average         1.6 0.8 0.1 0.0 0.0 0.0 0.0 2.5 0.05 0.04 4.50 3.25
± SEM                         0.01 0.01 1.12 0.92
 
Footnotes:
CRO: Corn Oil
DMBA: Dimethylbenzanthracene
IP/IJ: Intraperitoneal injection
INHAL: Inhalation