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Study Information
Study Attribute Value
CEBS Accession Number: 002-02466-0006-0000-6
Chemical Name: Methylenedianiline
CASRN: 101-77-9
NTP Study Type: Genetic Toxicology - Rodent Cytogenetics
NTP Study ID: 535562_CA
Study Result: Positive


Table Instructions and Notes:

Below are the detailed Chromosome Abberation data. You may also view the summary data for this study.


Initial Treatment Date Tissue Sample Time (h) Sex Species Strain Protocol Result
08/31/1988 Bone Marrow 17 Male Mouse B6C3F1 Intraperitoneal Injection x 1/study, 17 hour Positive

  Dose
(mg/kg)		 Animal
Number		 No.
Cells		 Chromatid Isochromatid Exchanges Aberrations
Breaks Gaps Breaks Gaps 'Tid 'Some Other Total Per Cell % Cells w/ Abs
(+ Gaps) (- Gaps) (+ Gaps) (- Gaps)
Positive Control Dimethylbenzanthracene 
50         
 8743 50 61 8 0 0 0 0 0 69 1.38 1.22 48.00 42.00
    8744 50 77 11 0 0 0 0 0 88 1.76 1.54 44.00 40.00
    8745 50 4 1 0 0 0 0 0 5 0.10 0.08 8.00 6.00
    8746 50 14 8 1 1 0 0 0 24 0.48 0.30 36.00 26.00
    8747 50 14 9 0 0 0 0 0 23 0.46 0.28 30.00 24.00
    8748 50 16 8 0 0 0 0 0 24 0.48 0.32 28.00 24.00
    8749 50 13 5 1 0 0 0 0 19 0.38 0.28 26.00 18.00
    8750 50 14 5 0 0 0 0 0 19 0.38 0.28 18.00 14.00
Average         26.6 6.9 0.3 0.1 0.0 0.0 0.0 33.9 0.68 0.54 29.75 24.25
± SEM                         0.20 0.19 4.64 4.32
 
Test Chemical Methylenedianiline 
50         
 8693 50 2 5 0 0 0 0 0 7 0.14 0.04 14.00 4.00
    8694 50 8 4 0 0 0 0 0 12 0.24 0.16 22.00 16.00
    8695 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    8696 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    8697 50 2 1 0 0 0 0 0 3 0.06 0.04 4.00 4.00
    8698 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    8699 50 3 1 0 0 0 0 0 4 0.08 0.06 8.00 6.00
    8700 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
Average         2.1 1.5 0.0 0.0 0.0 0.0 0.0 3.6 0.07 0.04 6.75 4.25
± SEM                         0.03 0.02 2.72 1.87
 
Test Chemical Methylenedianiline 
100         
 8703 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    8704 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    8705 50 1 2 0 0 0 0 0 3 0.06 0.02 6.00 2.00
    8706 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    8707 50 3 0 0 0 0 0 0 3 0.06 0.06 6.00 6.00
    8708 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    8709 50 2 1 0 0 0 0 0 3 0.06 0.04 6.00 4.00
    8710 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
Average         1.3 0.4 0.0 0.0 0.0 0.0 0.0 1.6 0.03 0.03 3.25 2.50
± SEM                         0.01 0.01 0.92 0.73
 
Test Chemical Methylenedianiline 
200         
 8713 50 5 3 0 0 0 0 0 8 0.16 0.10 16.00 10.00
    8714 50 7 2 0 0 0 0 0 9 0.18 0.14 16.00 14.00
    8715 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    8716 50 2 1 0 0 0 0 0 3 0.06 0.04 6.00 4.00
    8717 50 4 1 0 0 0 0 0 5 0.10 0.08 10.00 8.00
    8718 50 2 3 0 0 0 0 0 5 0.10 0.04 6.00 4.00
    8719 50 4 2 0 0 0 0 0 6 0.12 0.08 12.00 8.00
    8720 50 8 4 1 0 0 0 0 13 0.26 0.18 20.00 16.00
Average         4.0 2.1 0.1 0.0 0.0 0.0 0.0 6.3 0.13 0.08 11.00 8.00
± SEM                         0.03 0.02 2.17 1.89
 
Vehicle Control No Stressor 
 0         
 8733 50 3 0 0 0 0 0 0 3 0.06 0.06 6.00 6.00
    8734 50 2 1 0 0 0 0 0 3 0.06 0.04 6.00 4.00
    8735 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    8736 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    8737 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    8739 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    8740 50 4 0 0 0 0 0 0 4 0.08 0.08 8.00 8.00
    8741 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
Average         1.5 0.5 0.0 0.0 0.0 0.0 0.0 2.0 0.04 0.03 4.00 3.00
± SEM                         0.01 0.01 0.93 1.00
 
Footnotes:
CRO: Corn Oil
DMBA: Dimethylbenzanthracene
IP/IJ: Intraperitoneal injection
INHAL: Inhalation