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Study Information
Study Attribute Value
CEBS Accession Number: 002-02466-0006-0000-6
Chemical Name: Methylenedianiline
CASRN: 101-77-9
NTP Study Type: Genetic Toxicology - Rodent Cytogenetics
NTP Study ID: 535562_CA
Study Result: Positive


Table Instructions and Notes:

Below are the detailed Chromosome Abberation data. You may also view the summary data for this study.


Initial Treatment Date Tissue Sample Time (h) Sex Species Strain Protocol Result
02/07/1989 Bone Marrow 17 Male Mouse B6C3F1 Intraperitoneal Injection x 1/study, 17 hour Positive

  Dose
(mg/kg)		 Animal
Number		 No.
Cells		 Chromatid Isochromatid Exchanges Aberrations
Breaks Gaps Breaks Gaps 'Tid 'Some Other Total Per Cell % Cells w/ Abs
(+ Gaps) (- Gaps) (+ Gaps) (- Gaps)
Positive Control Dimethylbenzanthracene 
50         
 9747 50 34 21 0 0 0 0 0 55 1.10 0.68 44.00 38.00
    9748 50 21 15 4 1 0 0 1 42 0.84 0.52 36.00 28.00
    9749 50 40 13 2 0 0 0 0 55 1.10 0.84 50.00 46.00
    9750 50 0 2 0 0 0 0 0 2 0.04 0.00 4.00 0.00
    9751 50 25 9 2 0 0 0 0 36 0.72 0.54 46.00 38.00
    9752 50 8 2 1 0 0 0 0 11 0.22 0.18 8.00 4.00
    9753 50 9 2 1 0 0 0 0 12 0.24 0.20 18.00 16.00
    9754 50 6 2 0 0 0 0 0 8 0.16 0.12 18.00 14.00
Average         17.9 8.3 1.3 0.1 0.0 0.0 0.1 27.6 0.55 0.39 28.00 23.00
± SEM                         0.15 0.11 6.41 6.01
 
Test Chemical Methylenedianiline 
100         
 9677 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    9678 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    9679 50 0 1 1 0 0 0 0 2 0.04 0.02 4.00 2.00
    9680 50 3 0 0 0 0 0 0 3 0.06 0.06 6.00 6.00
    9681 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    9682 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    9683 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    9684 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
Average         0.9 0.6 0.1 0.0 0.0 0.0 0.0 1.6 0.03 0.02 3.25 2.00
± SEM                         0.01 0.01 0.65 0.65
 
Test Chemical Methylenedianiline 
150         
 9687 50 1 2 0 0 0 1 0 4 0.08 0.04 8.00 4.00
    9688 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    9689 50 8 6 0 0 0 0 0 14 0.28 0.16 12.00 12.00
    9690 50 7 6 1 0 0 0 0 14 0.28 0.16 22.00 12.00
    9691 50 0 2 0 0 0 0 0 2 0.04 0.00 4.00 0.00
    9692 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    9693 50 1 2 0 0 0 0 0 3 0.06 0.02 6.00 2.00
    9694 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
Average         2.4 2.3 0.1 0.0 0.0 0.1 0.0 4.9 0.10 0.05 7.00 4.25
± SEM                         0.04 0.02 2.56 1.79
 
Test Chemical Methylenedianiline 
200         
 9697 50 12 7 0 0 0 0 0 19 0.38 0.24 32.00 22.00
    9698 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    9699 50 1 2 0 0 0 0 0 3 0.06 0.02 6.00 2.00
    9700 50 8 5 1 0 0 0 0 14 0.28 0.18 22.00 16.00
    9701 50 2 3 0 0 0 0 0 5 0.10 0.04 10.00 4.00
    9702 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    9703 50 12 4 0 0 0 0 0 16 0.32 0.24 18.00 14.00
    9704 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
Average         4.5 2.6 0.1 0.0 0.0 0.0 0.0 7.3 0.15 0.09 11.25 7.50
± SEM                         0.06 0.04 4.14 3.02
 
Vehicle Control No Stressor 
 0         
 9737 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    9739 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    9740 50 2 2 0 0 0 0 0 4 0.08 0.04 2.00 2.00
    9741 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    9742 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    9743 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    9744 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    9745 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
Average         0.5 0.6 0.0 0.0 0.0 0.0 0.0 1.1 0.02 0.01 1.50 0.75
± SEM                         0.01 0.01 0.50 0.53
 
Footnotes:
CRO: Corn Oil
DMBA: Dimethylbenzanthracene
IP/IJ: Intraperitoneal injection
INHAL: Inhalation