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Study Information
Study Attribute Value
CEBS Accession Number: 002-01515-0014-0000-9
Chemical Name: 4-Nitro-o-phenylenediamine
CASRN: 99-56-9
NTP Study Type: Genetic Toxicology - Rodent Cytogenetics
NTP Study ID: 704945_CA
Study Result: Negative (Nonstandard Protocol)


Table Instructions and Notes:

Below are the detailed Chromosome Abberation data. You may also view the summary data for this study.


Initial Treatment Date Tissue Sample Time (h) Sex Species Strain Protocol Result
06/28/1984 Bone Marrow 17 Male Mouse B6C3F1 Intraperitoneal Injection x 1/study, 17 hour Negative (Nonstandard Protocol)

  Dose
(mg/kg)		 Animal
Number		 No.
Cells		 Chromatid Isochromatid Exchanges Aberrations
Breaks Gaps Breaks Gaps 'Tid 'Some Other Total Per Cell % Cells w/ Abs
(+ Gaps) (- Gaps) (+ Gaps) (- Gaps)
Positive Control Dimethylbenzanthracene 
200         
 1633 50 5 4 0 0 0 0 1 10 0.20 0.12 16.00 10.00
    1634 50 7 5 1 0 0 0 0 13 0.26 0.16 18.00 12.00
    1635 50 12 0 1 0 0 0 1 14 0.28 0.28 24.00 24.00
    1636 50 19 1 1 0 0 0 2 23 0.46 0.44 24.00 24.00
    1637 50 10 5 1 0 0 0 0 16 0.32 0.22 14.00 10.00
    1638 50 20 12 0 0 0 0 8 40 0.80 0.56 40.00 36.00
    1639 50 10 4 0 0 0 0 1 15 0.30 0.22 24.00 20.00
    1640 50 6 12 1 0 0 0 0 19 0.38 0.14 28.00 14.00
Average         11.1 5.4 0.6 0.0 0.0 0.0 1.6 18.8 0.38 0.27 23.50 18.75
± SEM                         0.07 0.06 2.90 3.21
 
Test Chemical 4-Nitro-o-phenylenediamine 
250         
 1665 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    1666 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    1667 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    1668 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    1669 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    1670 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    1671 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    1672 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
Average         0.8 0.0 0.0 0.0 0.0 0.0 0.0 0.8 0.02 0.02 1.50 1.50
± SEM                         0.01 0.01 0.50 0.50
 
Test Chemical 4-Nitro-o-phenylenediamine 
500         
 1673 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    1674 50 1 4 0 0 0 0 0 5 0.10 0.02 10.00 2.00
    1675 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    1676 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    1677 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    1678 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    1679 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    1680 50 3 1 0 0 0 0 1 5 0.10 0.08 8.00 6.00
Average         0.8 0.9 0.0 0.0 0.0 0.0 0.1 1.8 0.04 0.02 3.25 1.50
± SEM                         0.01 0.01 1.31 0.73
 
Test Chemical 4-Nitro-o-phenylenediamine 
1000         
 1681 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    1682 50 1 0 0 0 0 0 1 2 0.04 0.04 2.00 2.00
    1683 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    1684 50 2 0 1 1 0 0 0 4 0.08 0.06 4.00 2.00
    1685 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    1687 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    1688 50 2 2 0 0 0 0 0 4 0.08 0.04 8.00 4.00
    1689 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
Average         1.0 0.6 0.1 0.1 0.0 0.0 0.1 2.0 0.04 0.03 3.25 1.75
± SEM                         0.01 0.01 0.84 0.59
 
Vehicle Control No Stressor 
 0         
 1715 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    1716 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    1717 50 3 0 1 0 0 0 1 5 0.10 0.10 10.00 10.00
    1718 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    1719 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    1720 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    1721 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    1722 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
Average         0.8 0.0 0.1 0.0 0.0 0.0 0.1 1.0 0.02 0.02 2.00 2.00
± SEM                         0.01 0.01 1.25 1.25
 
Footnotes:
CRO: Corn Oil
DMBA: Dimethylbenzanthracene
IP/IJ: Intraperitoneal injection
INHAL: Inhalation