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Study Information
Study Attribute Value
CEBS Accession Number: 002-01694-0016-0000-9
Chemical Name: Benzidine dihydrochloride
CASRN: 531-85-1
NTP Study Type: Genetic Toxicology - Rodent Cytogenetics
NTP Study ID: 758694_CA
Study Result: Positive


Table Instructions and Notes:

Below are the detailed Chromosome Abberation data. You may also view the summary data for this study.


Initial Treatment Date Tissue Sample Time (h) Sex Species Strain Protocol Result
01/28/1985 Bone Marrow 17 Male Mouse B6C3F1 Intraperitoneal Injection x 1/study, 17 hour Positive

  Dose
(mg/kg)		 Animal
Number		 No.
Cells		 Chromatid Isochromatid Exchanges Aberrations
Breaks Gaps Breaks Gaps 'Tid 'Some Other Total Per Cell % Cells w/ Abs
(+ Gaps) (- Gaps) (+ Gaps) (- Gaps)
Positive Control Dimethylbenzanthracene 
100         
 1923 50 7 2 1 0 0 0 1 11 0.22 0.18 16.00 14.00
    1924 50 7 0 0 0 0 0 0 7 0.14 0.14 12.00 12.00
    1925 50 14 3 0 0 0 0 0 17 0.34 0.28 22.00 20.00
    1926 50 8 1 0 0 0 0 0 9 0.18 0.16 18.00 16.00
    1927 50 10 0 1 0 0 0 0 11 0.22 0.22 22.00 22.00
    1928 50 14 2 0 0 0 0 1 17 0.34 0.30 26.00 24.00
    1929 50 9 1 0 0 0 0 0 10 0.20 0.18 20.00 18.00
    1930 50 2 3 1 0 0 0 1 7 0.14 0.08 14.00 8.00
Average         8.9 1.5 0.4 0.0 0.0 0.0 0.4 11.1 0.22 0.19 18.75 16.75
± SEM                         0.03 0.03 1.64 1.89
 
Test Chemical Benzidine dihydrochloride 
50         
 1953 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    1954 50 2 0 0 0 0 0 0 2 0.04 0.04 2.00 2.00
    1955 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    1956 50 3 0 0 0 1 0 0 4 0.08 0.08 8.00 8.00
    1957 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    1958 50 3 0 0 0 0 0 0 3 0.06 0.06 6.00 6.00
    1959 50 5 0 0 0 0 0 0 5 0.10 0.10 10.00 10.00
    1960 50 4 1 0 0 0 0 0 5 0.10 0.08 8.00 6.00
Average         2.6 0.1 0.0 0.0 0.1 0.0 0.0 2.9 0.06 0.06 5.25 5.00
± SEM                         0.01 0.01 1.13 1.07
 
Test Chemical Benzidine dihydrochloride 
100         
 1943 50 4 0 0 0 0 0 0 4 0.08 0.08 8.00 8.00
    1944 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    1945 50 3 0 0 0 0 0 0 3 0.06 0.06 6.00 6.00
    1946 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    1947 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    1948 50 5 0 0 0 0 0 0 5 0.10 0.10 10.00 10.00
    1949 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    1950 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
Average         2.3 0.1 0.0 0.0 0.0 0.0 0.0 2.4 0.05 0.05 4.75 4.50
± SEM                         0.01 0.01 1.13 1.18
 
Test Chemical Benzidine dihydrochloride 
200         
 1933 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    1934 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    1935 50 3 0 0 0 0 0 0 3 0.06 0.06 6.00 6.00
    1936 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    1937 50 1 2 0 0 0 0 0 3 0.06 0.02 6.00 2.00
    1938 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    1939 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    1940 50 2 1 0 0 0 0 0 3 0.06 0.04 6.00 4.00
Average         1.1 0.8 0.0 0.0 0.0 0.0 0.0 1.9 0.04 0.02 3.75 2.25
± SEM                         0.01 0.01 0.70 0.80
 
Vehicle Control No Stressor 
 0         
 1963 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    1964 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    1965 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    1966 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    1967 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    1968 50 3 0 0 0 0 0 0 3 0.06 0.06 6.00 6.00
    1969 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    1970 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
Average         1.3 0.0 0.0 0.0 0.0 0.0 0.0 1.3 0.03 0.03 2.50 2.50
± SEM                         0.01 0.01 0.73 0.73
 
Footnotes:
CRO: Corn Oil
DMBA: Dimethylbenzanthracene
IP/IJ: Intraperitoneal injection
INHAL: Inhalation