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Study Information
Study Attribute Value
CEBS Accession Number: 002-01694-0016-0000-9
Chemical Name: Benzidine dihydrochloride
CASRN: 531-85-1
NTP Study Type: Genetic Toxicology - Rodent Cytogenetics
NTP Study ID: 758694_CA
Study Result: Positive


Table Instructions and Notes:

Below are the detailed Chromosome Abberation data. You may also view the summary data for this study.


Initial Treatment Date Tissue Sample Time (h) Sex Species Strain Protocol Result
01/21/1986 Bone Marrow 17 Male Mouse B6C3F1 Intraperitoneal Injection x 1/study, 17 hour Positive

  Dose
(mg/kg)		 Animal
Number		 No.
Cells		 Chromatid Isochromatid Exchanges Aberrations
Breaks Gaps Breaks Gaps 'Tid 'Some Other Total Per Cell % Cells w/ Abs
(+ Gaps) (- Gaps) (+ Gaps) (- Gaps)
Positive Control Dimethylbenzanthracene 
100         
 3080 50 20 4 0 0 0 0 0 24 0.48 0.40 36.00 30.00
    3081 50 8 9 0 0 0 0 0 17 0.34 0.16 22.00 16.00
    3082 50 10 6 0 0 0 0 0 16 0.32 0.20 22.00 16.00
    3083 50 9 2 0 2 0 0 0 13 0.26 0.18 20.00 14.00
    3084 50 16 8 0 0 0 0 0 24 0.48 0.32 24.00 20.00
    3085 50 20 8 0 1 0 0 0 29 0.58 0.40 32.00 26.00
    3086 50 15 10 0 0 0 0 0 25 0.50 0.30 24.00 20.00
    3087 50 12 7 0 1 0 0 0 20 0.40 0.24 20.00 16.00
Average         13.8 6.8 0.0 0.5 0.0 0.0 0.0 21.0 0.42 0.28 25.00 19.75
± SEM                         0.04 0.03 2.07 1.98
 
Test Chemical Benzidine dihydrochloride 
50         
 3040 50 0 1 0 1 0 0 0 2 0.04 0.00 4.00 0.00
    3041 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3042 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3043 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3044 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    3045 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3046 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    3047 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
Average         0.4 0.4 0.0 0.1 0.0 0.0 0.0 0.9 0.02 0.01 1.75 0.75
± SEM                         0.01 0.00 0.70 0.37
 
Test Chemical Benzidine dihydrochloride 
100         
 3050 50 3 1 0 0 0 0 0 4 0.08 0.06 8.00 6.00
    3051 50 3 0 0 0 0 0 0 3 0.06 0.06 6.00 6.00
    3052 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3053 50 2 1 0 0 0 0 0 3 0.06 0.04 4.00 4.00
    3054 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3055 50 4 1 0 0 0 0 0 5 0.10 0.08 10.00 8.00
    3056 50 2 1 0 0 0 0 0 3 0.06 0.04 6.00 4.00
    3057 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
Average         1.8 0.5 0.0 0.0 0.0 0.0 0.0 2.3 0.05 0.04 4.25 3.50
± SEM                         0.01 0.01 1.39 1.12
 
Test Chemical Benzidine dihydrochloride 
200         
 3060 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3061 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3062 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    3063 50 2 0 0 2 0 0 1 5 0.10 0.06 8.00 6.00
    3064 50 0 0 1 0 0 0 0 1 0.02 0.02 2.00 2.00
    3065 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3066 50 1 3 0 0 0 0 0 4 0.08 0.02 6.00 2.00
    3067 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
Average         0.6 0.4 0.1 0.3 0.0 0.0 0.1 1.5 0.03 0.02 2.50 1.75
± SEM                         0.01 0.01 1.05 0.70
 
Vehicle Control No Stressor 
 0         
 3070 50 2 0 0 0 0 0 1 3 0.06 0.06 4.00 4.00
    3071 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    3072 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    3073 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3074 50 3 1 0 0 0 0 0 4 0.08 0.06 8.00 6.00
    3075 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    3076 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    3077 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
Average         1.0 0.3 0.0 0.0 0.0 0.0 0.1 1.4 0.03 0.02 2.50 2.00
± SEM                         0.01 0.01 0.91 0.76
 
Footnotes:
CRO: Corn Oil
DMBA: Dimethylbenzanthracene
IP/IJ: Intraperitoneal injection
INHAL: Inhalation