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Study Information
Study Attribute Value
CEBS Accession Number: 002-01694-0016-0000-9
Chemical Name: Benzidine dihydrochloride
CASRN: 531-85-1
NTP Study Type: Genetic Toxicology - Rodent Cytogenetics
NTP Study ID: 758694_CA
Study Result: Positive


Table Instructions and Notes:

Below are the detailed Chromosome Abberation data. You may also view the summary data for this study.


Initial Treatment Date Tissue Sample Time (h) Sex Species Strain Protocol Result
06/24/1987 Bone Marrow 36 Male Mouse B6C3F1 Intraperitoneal Injection x 1/study, 36 hour Positive

  Dose
(mg/kg)		 Animal
Number		 No.
Cells		 Chromatid Isochromatid Exchanges Aberrations
Breaks Gaps Breaks Gaps 'Tid 'Some Other Total Per Cell % Cells w/ Abs
(+ Gaps) (- Gaps) (+ Gaps) (- Gaps)
Positive Control Dimethylbenzanthracene 
100         
 6008 50 21 10 3 0 0 0 0 34 0.68 0.48 32.00 28.00
    6009 50 13 4 3 0 0 0 0 20 0.40 0.32 28.00 24.00
    6010 50 10 15 3 0 0 0 0 28 0.56 0.26 32.00 20.00
    6011 50 23 14 3 0 0 0 0 40 0.80 0.52 40.00 34.00
    6012 50 9 8 4 0 0 0 0 21 0.42 0.26 30.00 20.00
    6013 50 8 4 1 0 0 0 0 13 0.26 0.18 22.00 16.00
    6014 50 11 19 1 1 0 0 0 32 0.64 0.24 38.00 20.00
    6015 50 14 6 4 0 0 0 0 24 0.48 0.36 32.00 26.00
Average         13.6 10.0 2.8 0.1 0.0 0.0 0.0 26.5 0.53 0.33 31.75 23.50
± SEM                         0.06 0.04 1.98 2.03
 
Test Chemical Benzidine dihydrochloride 
50         
 5928 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    5929 50 3 3 1 0 0 0 0 7 0.14 0.08 14.00 8.00
    5930 50 3 1 0 1 0 0 0 5 0.10 0.06 8.00 6.00
    5931 50 1 3 0 0 0 0 0 4 0.08 0.02 6.00 2.00
    5932 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    5933 50 1 2 0 0 0 0 0 3 0.06 0.02 6.00 2.00
    5934 50 3 1 0 0 0 0 0 4 0.08 0.06 6.00 4.00
    5935 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
Average         1.9 1.4 0.1 0.1 0.0 0.0 0.0 3.5 0.07 0.04 6.25 3.75
± SEM                         0.01 0.01 1.28 0.88
 
Test Chemical Benzidine dihydrochloride 
100         
 5938 50 3 1 0 0 0 0 0 4 0.08 0.06 8.00 6.00
    5939 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    5940 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    5941 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    5942 50 2 2 0 0 0 0 0 4 0.08 0.04 8.00 4.00
    5943 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    5944 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    5945 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
Average         1.4 0.9 0.0 0.0 0.0 0.0 0.0 2.3 0.05 0.03 4.50 2.75
± SEM                         0.01 0.01 0.91 0.65
 
Test Chemical Benzidine dihydrochloride 
200         
 5948 50 3 0 1 1 0 0 0 5 0.10 0.08 10.00 8.00
    5949 50 0 2 0 0 0 0 0 2 0.04 0.00 4.00 0.00
    5950 50 5 1 2 0 0 0 0 8 0.16 0.14 16.00 14.00
    5951 50 3 0 0 0 0 0 0 3 0.06 0.06 6.00 6.00
    5952 50 6 1 1 0 0 0 0 8 0.16 0.14 16.00 14.00
    5953 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    5954 50 1 2 0 0 0 0 0 3 0.06 0.02 6.00 2.00
    5955 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
Average         2.4 0.8 0.5 0.1 0.0 0.0 0.0 3.8 0.08 0.06 7.50 5.75
± SEM                         0.02 0.02 2.13 2.05
 
Vehicle Control No Stressor 
 0         
 5988 50 3 0 0 0 0 0 0 3 0.06 0.06 6.00 6.00
    5989 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    5990 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
    5991 50 2 0 0 0 0 0 0 2 0.04 0.04 4.00 4.00
    5992 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    5993 50 1 0 0 0 0 0 0 1 0.02 0.02 2.00 2.00
    5994 50 1 2 0 0 0 0 0 3 0.06 0.02 4.00 2.00
    5995 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
Average         0.9 0.4 0.0 0.0 0.0 0.0 0.0 1.3 0.03 0.02 2.25 1.75
± SEM                         0.01 0.01 0.80 0.80
 
Footnotes:
CRO: Corn Oil
DMBA: Dimethylbenzanthracene
IP/IJ: Intraperitoneal injection
INHAL: Inhalation