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Study Information
Study Attribute Value
CEBS Accession Number: 002-01694-0016-0000-9
Chemical Name: Benzidine dihydrochloride
CASRN: 531-85-1
NTP Study Type: Genetic Toxicology - Rodent Cytogenetics
NTP Study ID: 758694_CA
Study Result: Positive


Table Instructions and Notes:

Below are the detailed Chromosome Abberation data. You may also view the summary data for this study.


Initial Treatment Date Tissue Sample Time (h) Sex Species Strain Protocol Result
03/14/1989 Bone Marrow 36 Male Mouse B6C3F1 Intraperitoneal Injection x 1/study, 36 hour Positive

  Dose
(mg/kg)		 Animal
Number		 No.
Cells		 Chromatid Isochromatid Exchanges Aberrations
Breaks Gaps Breaks Gaps 'Tid 'Some Other Total Per Cell % Cells w/ Abs
(+ Gaps) (- Gaps) (+ Gaps) (- Gaps)
Positive Control Dimethylbenzanthracene 
50         
 9922 50 32 9 0 0 0 0 0 41 0.82 0.64 20.00 16.00
    9923 50 11 9 0 0 0 0 0 20 0.40 0.22 24.00 20.00
    9924 50 18 13 0 0 0 0 0 31 0.62 0.36 42.00 38.00
    9925 50 13 17 3 4 0 0 0 37 0.74 0.32 40.00 32.00
    9926 50 10 7 0 0 0 0 0 17 0.34 0.20 24.00 18.00
    9927 50 22 16 1 0 0 0 1 40 0.80 0.48 40.00 32.00
    9928 50 14 10 1 0 1 0 0 26 0.52 0.32 30.00 24.00
    9929 50 4 6 0 0 0 0 0 10 0.20 0.08 14.00 6.00
Average         15.5 10.9 0.6 0.5 0.1 0.0 0.1 27.8 0.56 0.33 29.25 23.25
± SEM                         0.08 0.06 3.70 3.68
 
Test Chemical Benzidine dihydrochloride 
100         
 9883 50 4 7 1 0 0 0 0 12 0.24 0.10 14.00 6.00
    9885 50 2 2 1 0 0 0 0 5 0.10 0.06 8.00 4.00
    9886 50 1 0 1 0 0 0 0 2 0.04 0.04 4.00 4.00
    9887 50 3 0 0 0 0 0 0 3 0.06 0.06 6.00 6.00
    9888 50 2 3 2 0 0 0 0 7 0.14 0.08 10.00 6.00
    9889 50 3 2 0 0 0 0 0 5 0.10 0.06 8.00 4.00
    9890 50 0 1 1 0 0 0 0 2 0.04 0.02 4.00 2.00
    9891 50 2 0 0 0 0 0 0 2 0.04 0.04 2.00 2.00
Average         2.1 1.9 0.8 0.0 0.0 0.0 0.0 4.8 0.10 0.06 7.00 4.25
± SEM                         0.02 0.01 1.36 0.59
 
Test Chemical Benzidine dihydrochloride 
150         
 9892 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    9893 50 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00
    9894 50 2 1 0 0 0 0 0 3 0.06 0.04 6.00 4.00
    9895 50 6 0 1 0 0 0 0 7 0.14 0.14 8.00 8.00
    9896 50 2 1 0 0 0 0 0 3 0.06 0.04 6.00 4.00
    9897 50 3 9 0 0 0 0 0 12 0.24 0.06 16.00 6.00
    9898 50 3 9 0 0 0 0 0 12 0.24 0.06 20.00 8.00
    9899 50 4 2 0 0 0 0 0 6 0.12 0.08 6.00 4.00
Average         2.5 2.8 0.1 0.0 0.0 0.0 0.0 5.4 0.11 0.05 7.75 4.25
± SEM                         0.03 0.02 2.49 1.10
 
Test Chemical Benzidine dihydrochloride 
200         
 9902 50 6 6 0 0 0 0 0 12 0.24 0.12 18.00 12.00
    9903 50 1 1 0 0 0 0 2 4 0.08 0.06 8.00 6.00
    9904 50 9 5 1 0 0 0 0 15 0.30 0.20 26.00 18.00
    9905 50 4 6 2 0 0 0 0 12 0.24 0.12 12.00 6.00
    9906 50 3 3 0 0 0 0 0 6 0.12 0.06 12.00 6.00
    9907 50 13 8 0 0 0 0 3 24 0.48 0.32 36.00 26.00
    9908 50 0 2 0 0 0 0 0 2 0.04 0.00 4.00 0.00
    9909 50 0 1 0 0 0 0 0 1 0.02 0.00 2.00 0.00
Average         4.5 4.0 0.4 0.0 0.0 0.0 0.6 9.5 0.19 0.11 14.75 9.25
± SEM                         0.06 0.04 4.07 3.18
 
Vehicle Control No Stressor 
 0         
 9912 50 2 1 0 0 0 0 0 3 0.06 0.04 4.00 2.00
    9913 50 7 2 0 0 0 0 0 9 0.18 0.14 12.00 10.00
    9914 50 2 1 0 0 0 0 0 3 0.06 0.04 6.00 4.00
    9915 50 0 1 0 0 0 0 1 2 0.04 0.02 4.00 2.00
    9916 50 5 4 0 0 0 0 0 9 0.18 0.10 14.00 8.00
    9917 50 2 0 0 0 0 0 0 2 0.04 0.04 2.00 2.00
    9918 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
    9919 50 1 1 0 0 0 0 0 2 0.04 0.02 4.00 2.00
Average         2.5 1.4 0.0 0.0 0.0 0.0 0.1 4.0 0.08 0.05 6.25 4.00
± SEM                         0.02 0.02 1.53 1.13
 
Footnotes:
CRO: Corn Oil
DMBA: Dimethylbenzanthracene
IP/IJ: Intraperitoneal injection
INHAL: Inhalation