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Principal Investigator: Moraes, Carlos Torres
Institute Receiving Award University Of Miami School Of Medicine
Location Coral Gables, FL
Grant Number R33ES025673
Funding Organization National Institute of Environmental Health Sciences
Award Funding Period 15 Sep 2018 to 31 Aug 2021
DESCRIPTION (provided by applicant):  : Imaging mitochondrial signaling in beta cells (β cells) ectopically implanted in the eye. In the last 20 years, it became clear that defects in the mitochondrial energy producing system, either genetic or toxin-induced, cause many different phenotypes. Therefore, defects in one of the five mitochondrial oxidative phosphorylation (OXPHOS) complexes likely trigger distinct signaling pathways, which differentially affect specific cell types. This team will use mouse models with specific defects in complexes I, III or IV to explore enzyme-specific signaling events in mitochondrial disorders. A novel model will be used to test this hypothesis in a dynamic, real-time platform. The approach will involve implanting β cells in the anterior chamber of the mouse eye, which allows for the use of imaging techniques to follow both cellular and mitochondrial function as well as its signaling patterns in vascularized environment akin to the in vivo situation. This is a multiple Principal Investigator application that will use the expertise of the group of Dr. Alejandro Caicedo (β-cell implant model, imaging physiological biomarkers) and the group of Dr. Carlos Moraes (mitochondrial physiology and mouse models of OXPHOS defects) to tease out signaling signatures associated with defects (genetic or toxin-induced) in specific OXPHOS complexes.
Science Code(s)/Area of Science(s) Primary: 64 - Mitochondrial Disorders
Publications See publications associated with this Grant.
Program Officer Daniel Shaughnessy
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