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National Institute of Environmental Health Sciences

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Publication Detail

Title: Construction of an Escherichia coli K-12 ada deletion by gene replacement in a recD strain reveals a second methyltransferase that repairs alkylated DNA.

Authors: Shevell, D E; Abou-Zamzam, A M; Demple, B; Walker, G C

Published In J Bacteriol, (1988 Jul)

Abstract: We constructed an ada deletion by gene replacement in a recD1014 strain of Escherichia coli. Characterization of a delta ada-25 recD+ strain revealed the presence of a second DNA methyltransferase activity in E. coli K-12 which transfers a methyl group from methylated DNA to a protein with a molecular weight of 18,000 to 20,000.

PubMed ID: 3290202 Exiting the NIEHS site

MeSH Terms: Alkylation; Bacterial Proteins/genetics*; Chromosome Deletion; DNA Repair*; DNA, Bacterial/metabolism*; Escherichia coli Proteins*; Escherichia coli/enzymology; Escherichia coli/genetics*; Genes, Bacterial; Methylation; Methyltransferases/metabolism*; Mutation; O(6)-Methylguanine-DNA Methyltransferase; Transcription Factors

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