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Title: Radial capillary array electrophoresis microplate and scanner for high-performance nucleic acid analysis.

Authors: Shi, Y; Simpson, P C; Scherer, J R; Wexler, D; Skibola, C; Smith, M T; Mathies, R A

Published In Anal Chem, (1999 Dec 01)

Abstract: The design, fabrication, and operation of a radial capillary array electrophoresis microplate and scanner for high-throughput DNA analysis is presented. The microplate consists of a central common anode reservoir coupled to 96 separate microfabricated separation channels connected to sample injectors on the perimeter of the 10-cm-diameter wafer. Detection is accomplished by a laser-excited rotary confocal scanner with four color detection channels. Loading of 96 samples in parallel is achieved using a pressurized capillary array system. High-quality separations of 96 pBR322 restriction digest samples are achieved in < 120 s with the microplate system. The practical utility and multicolor detection capability is demonstrated by analyzing 96 methylenetetrahydrofolate reductase (MTHFR) alleles in parallel using a noncovalent 2-color staining method. This work establishes the feasibility of performing high-throughput genotyping separations with capillary array electrophoresis microplates.

PubMed ID: 10596215 Exiting the NIEHS site

MeSH Terms: Electrophoresis, Capillary/methods*; Nucleic Acids/analysis*

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