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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Analysis of free malondialdehyde in photoirradiated corn oil and beef fat via a pyrazole derivative.

Authors: Umano, K; Dennis, K J; Shibamoto, T

Published In Lipids, (1988 Aug)

Abstract: Malondialdehyde (MA) formed in linolenic acid, linoleic acid, corn oil and beef fat upon photoirradiation was determined by gas chromatography (GC). The MA produced was reacted with methylhydrazine to give 1-methylpyrazole and was subsequently analyzed on a GC equipped with a nitrogen-phosphorus specific detector and a fused silica capillary column. MA values determined by this method correspond to free or unbound MA levels. Linolenic and linoleic acids produced 867 micrograms MA/g and 106 micrograms MA/g, respectively. Oleic and stearic acids did not produce detectable levels of MA upon photoirradiation. Amounts of MA produced after eight hour irradiations of corn oil and beef fat were 56.24 micrograms/g and 25.01 micrograms/g, respectively. Some photoreaction products in irradiated corn oil also were identified as methylhydrazine derivatives.

PubMed ID: 3185115 Exiting the NIEHS site

MeSH Terms: Calibration; Chromatography, Gas/instrumentation; Corn Oil/analysis*; Fats/analysis*; Hexanes; Malonates/analysis*; Malondialdehyde/analysis*; Methylhydrazines/analysis; Photolysis; Plant Oils/analysis*

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