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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Rhamnolipid (biosurfactant) effects on cell aggregation and biodegradation of residual hexadecane under saturated flow conditions.

Authors: Herman, D C; Zhang, Y; Miller, R M

Published In Appl Environ Microbiol, (1997 Sep)

Abstract: The objective of this research was to evaluate the effect of low concentrations of a rhamnolipid biosurfactant on the in situ biodegradation of hydrocarbon entrapped in a porous matrix. Experiments were performed with sand-packed columns under saturated flow conditions with hexadecane as a model hydrocarbon. Application of biosurfactant concentrations greater than the CMC (the concentration at which the surfactant molecules spontaneously form micelles or vesicles [0.03 mM]) resulted primarily in the mobilization of hexadecane entrapped within the sand matrix. In contrast, application of biosurfactant concentrations less than the CMC enhanced the in situ mineralization of entrapped hexadecane; however, this effect was dependent on the choice of bacterial isolate. The two Pseudomonas isolates tested, R4 and ATCC 15524, were used because they exhibit different patterns of biodegradation of hexadecane, and they also differed in their physical response to rhamnolipid addition. ATCC 15524 cells formed extensive multicell aggregates in the presence of rhamnolipid while R4 cells were unaffected. This behavior did not affect the ability of the biosurfactant to enhance the biodegradation of hexadecane in well-mixed soil slurry systems but had a large affect on the extent of entrapped hexadecane biodegradation in the sand-packed-column system that was used in this study.

PubMed ID: 9293014 Exiting the NIEHS site

MeSH Terms: Alkanes/metabolism*; Bacterial Adhesion/drug effects; Bacteriological Techniques; Biodegradation, Environmental/drug effects; Glycolipids/biosynthesis; Glycolipids/chemistry; Glycolipids/pharmacology*; Pseudomonas aeruginosa/cytology; Pseudomonas aeruginosa/drug effects*; Pseudomonas aeruginosa/metabolism*; Rhamnose/chemistry; Soil Microbiology; Surface-Active Agents/chemistry; Surface-Active Agents/metabolism; Surface-Active Agents/pharmacology*

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