Title: Killing and mutation of human lymphoblast cells by aflatoxin B1: evidence for an inducible repair response.
Authors: Kaden, D A; Call, K M; Leong, P M; Komives, E A; Thilly, W G
Published In Cancer Res, (1987 Apr 15)
Abstract: Diploid human lymphoblast cells exhibit apparent saturation of mutation induced by exposure to aflatoxin B1, despite a linear increase in the amount and proportion of the aflatoxin-DNA adducts formed. The saturation is neither a cell cycle phenomenon nor a result of a genetically heterozygous population. Examination of the biphasic nature of aflatoxin-DNA adduct loss in vivo shows initial, rapid removal of all adduct species, followed by a slow loss of the aflatoxin-N7-guanine adduct alone. We hypothesize that these data reveal two modes of adduct loss in these cells. The first is an inducible, error-free system that is short-lived, turning off as adduct levels fall below the induction threshold of some 1000 total adducts/cell. The second loss is slower and results from spontaneous depurination of remaining aflatoxin-N7-guanines. Our data are in agreement with the possibility that apurinic sites thus generated are responsible for the mutation observed. A major paradox arises from the fact that aflatoxin-related premutagenic depurinations are estimated to be only 10% of the number of spontaneous depurinations estimated by others to occur in human cells in a 1-h period.
PubMed ID: 3103909
MeSH Terms: Aflatoxin B1; Aflatoxins/metabolism; Aflatoxins/toxicity*; Cell Cycle/drug effects; Cell Line; Cell Survival/drug effects; DNA Repair/drug effects*; DNA/metabolism; Humans; Lymphocytes/drug effects*; Mutation*; Time Factors