Title: The rat elastase I regulatory element is an enhancer that directs correct cell specificity and developmental onset of expression in transgenic mice.
Authors: Hammer, R E; Swift, G H; Ornitz, D M; Quaife, C J; Palmiter, R D; Brinster, R L; MacDonald, R J
Published In Mol Cell Biol, (1987 Aug)
Abstract: A total of 134 base pairs of the 5' flanking sequence of the elastase I gene is sufficient and necessary to direct expression of the passive human growth hormone gene (hGH) to the exocrine pancreas. We demonstrate that this elastase I regulatory region contains a transcriptional enhancer which directs acinar cell-specific expression in transgenic animals. The elastase I enhancer specifies correct expression of the linked hGH gene in an orientation- and position-independent manner and can activate a heterologous promoter. The enhancer also directs the appropriate temporal activation of the hGH gene in the developing pancreas. Transcription is initiated correctly for the elastase I or hGH promoter, and the transcripts are correctly processed regardless of the enhancer position within or outside the fusion gene. The elastase I enhancer generates coincident DNase I-hypersensitive sites in pancreatic chromatin when moved 3 kilobases upstream or within the first intron of the hGH gene and when associated with the hGH promoter.
PubMed ID: 3670302
MeSH Terms: Animals; Base Composition; Base Sequence; Enhancer Elements, Genetic*; Genes*; Genes, Regulator*; Growth Hormone/genetics; Humans; Hybridization, Genetic; Mice; Mice, Inbred Strains; Nucleic Acid Hybridization; Pancreatic Elastase/genetics*; Transcription, Genetic*