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Title: Maintenance of adult rat liver slices in dynamic organ culture.

Authors: Smith, P F; Krack, G; McKee, R L; Johnson, D G; Gandolfi, A J; Hruby, V J; Krumdieck, C L; Brendel, K

Published In In Vitro Cell Dev Biol, (1986 Dec)

Abstract: Adult rat liver slices were maintained for 20 h in a novel organ culture system with minimal loss of viability and function. Potassium and adenosine triphosphate levels were maintained at in vivo levels, following an initial recovery period (2 to 4 h), for up to 20 h. Protein synthesis and secretion were linear for 20 and 16 h, respectively. In addition, the liver slices synthesized glycogen between 4 and 12 h in culture. Finally, the liver slices were hormonally responsive during the 20 h culture period as exemplified by glucagon-stimulated glucose production. This system provides a simple and effective method for the culture and biochemical maintenance of adult rat liver for 20 h with minimal loss of biochemical function.

PubMed ID: 3782009 Exiting the NIEHS site

MeSH Terms: Adenosine Triphosphate/analysis; Animals; Glucose/biosynthesis; Liver Glycogen/analysis; Liver/analysis; Liver/metabolism; Liver/physiology*; Male; Organ Culture Techniques*; Potassium/analysis; Protein Biosynthesis; Rats; Rats, Inbred Strains

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