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Title: Creatinine measurements in 24 h urine by liquid chromatography--tandem Mass Spectrometry.

Authors: Park, Eun-Kee; Watanabe, Takaho; Gee, Shirley J; Schenker, Marc B; Hammock, Bruce D

Published In J Agric Food Chem, (2008 Jan 23)

Abstract: A simple, sensitive, and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determining urinary creatinine was developed and used to evaluate 24 h urine samples collected during an exposure study. Urine (1 microL) was diluted with methanol and then directly applied to LC-MS/MS. Under electrospray ionization (ESI) conditions, the transition molecules of creatinine and creatinine- d3 were observed at m/ z 114 > 44 and m/ z 117 > 47, respectively. The retention time of creatinine was 0.59 min. The linear range was 1-2000 ng/mL, with a detection limit in urine of 1 ng/mL. LC-MS/MS and colorimetric end-point methods were significantly associated ( R2 = 0.8785, p < 0.0001). The LC-MS/MS method to determine creatinine in 24 h urine samples had shorter retention times, was more sensitive, reliable, reproducible, simple, selective, and used a smaller sample size than other LC-MS/MS or commercial methods.

PubMed ID: 18092755 Exiting the NIEHS site

MeSH Terms: Chromatography, High Pressure Liquid*; Creatinine/urine*; Euryarchaeota; Reproducibility of Results; Sensitivity and Specificity; Tandem Mass Spectrometry*; Time Factors

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