Title: Encapsulation of methylene blue in polyacrylamide nanoparticle platforms protects its photodynamic effectiveness.
Authors: Tang, Wei; Xu, Hao; Park, Edwin J; Philbert, Martin A; Kopelman, Raoul
Published In Biochem Biophys Res Commun, (2008 May 02)
Abstract: The ability to prevent methylene blue (MB), a photosensitizer, from being reduced by plasma reductases will greatly improve its efficacy in photodynamic therapy (PDT) applications. We have developed a delivery approach for PDT by encapsulating MB using nanoparticle platforms (NPs). The 30-nm polyacrylamide-based NPs provide protection for the embedded MB against reduction by diaphorase enzymes. Furthermore, our data shows the matrix-protected MB efficiently induces photodynamic damage to tumor cells. The unprecedented results demonstrate the significant in vitro photodynamic effectiveness of MB when encapsulated within NPs, which promises to open new opportunities for MB in its in vivo and clinical studies.
PubMed ID: 18298950
MeSH Terms: Animals; Cell Line, Tumor; Drug Carriers/chemistry*; Glioma/pathology*; Glioma/therapy*; Methylene Blue/administration & dosage*; Methylene Blue/chemistry*; Nanoparticles/chemistry*; Nanoparticles/ultrastructure; Photochemotherapy/methods*; Rats