Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

National Institute of Environmental Health Sciences

Use this QR code to view the newest version of this document
Use this QR code to view the newest version of this document

Your Environment. Your Health.

Publication Detail

Title: Temporal transcriptomic microarray analysis of "Dehalococcoides ethenogenes" strain 195 during the transition into stationary phase.

Authors: Johnson, David R; Brodie, Eoin L; Hubbard, Alan E; Andersen, Gary L; Zinder, Stephen H; Alvarez-Cohen, Lisa

Published In Appl Environ Microbiol, (2008 May)

Abstract: "Dehalococcoides" bacteria can reductively dehalogenate a wide range of halogenated organic pollutants. In this study, DNA microarrays were used to monitor dynamic changes in the transcriptome as "Dehalococcoides ethenogenes" strain 195 transitioned from exponential growth into stationary phase. In total, 415 nonredundant genes were identified as differentially expressed. As expected, genes involved with translation and energy metabolism were down-regulated while genes involved with general stress response, transcription, and signal transduction were up-regulated. Unexpected, however, was the 8- to 10-fold up-regulation of four putative reductive dehalogenases (RDases) (DET0173, DET0180, DET1535, and DET1545). Another unexpected finding was the up-regulation of a large number of genes located within integrated elements, including a putative prophage and a multicopy transposon. Finally, genes encoding the dominant hydrogenase-RDase respiratory chain of this strain (Hup and TceA) were expressed at stable levels throughout the experiment, providing molecular evidence that strain 195 can uncouple dechlorination from net growth.

PubMed ID: 18310438 Exiting the NIEHS site

MeSH Terms: Bacterial Proteins/genetics; Chloroflexi/genetics*; Chloroflexi/growth & development*; DNA Transposable Elements; Down-Regulation; Energy Metabolism/genetics; Enzymes/genetics; Gene Expression Profiling*; Microarray Analysis*; Prophages/genetics; Protein Biosynthesis; Signal Transduction/genetics; Time Factors; Transcription Factors/genetics

Back
to Top