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Title: Quantitative detection of perchlorate-reducing bacteria by real-time PCR targeting the perchlorate reductase gene.

Authors: Nozawa-Inoue, Mamie; Jien, Mercy; Hamilton, Nicholas S; Stewart, Valley; Scow, Kate M; Hristova, Krassimira R

Published In Appl Environ Microbiol, (2008 Mar)

Abstract: A quantitative real-time PCR assay targeting the pcrA gene, encoding the catalytic subunit of perchlorate reductase, detected pcrA genes from perchlorate-reducing bacteria in three different genera and from soil microbial communities. Partial pcrA sequences indicated differences in the composition of perchlorate-reducing bacterial communities following exposure to different electron donors.

PubMed ID: 18245250 Exiting the NIEHS site

MeSH Terms: Amino Acid Sequence; Bacteria/classification; Bacteria/genetics*; Bacteria/metabolism; Bacterial Proteins/genetics*; Bacterial Proteins/metabolism; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; Molecular Sequence Data; Oxidation-Reduction; Oxidoreductases/genetics*; Oxidoreductases/metabolism; Perchloric Acid/metabolism*; Phylogeny; Polymerase Chain Reaction/methods*; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Soil Microbiology

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