Title: Human promyelocytic leukemia cells (HL-60): a new model to study the effects of chemopreventive agents on H2O2 production.
Authors: Bhimani, R S; Zhong, Z; Schleifer, E; Troll, W; Frenkel, K
Published In Cancer Detect Prev, (1995)
Abstract: Tumor promoter-stimulated polymorphonuclear leukocytes (PMNs) produce excessive H2O2, which contributes to inflammation and carcinogenesis. A new model to study 12-O-tetradecanoylphorbol-13-acetate (TPA)-mediated H2O2 formation and its suppression by chemopreventive agents was developed using human promyelocytic leukemic HL-60 cells and validated by comparing results with those obtained using human PMNs. Equal H2O2 (20 to 25 nmol/ml) was generated by TPA-activated PMNs (2.5 x 10(5)/ml) and TPA-treated dimethylsulfoxide (DMSO)-differentiated HL-60 cells (5 x 10(5)/ml). The chemopreventive agent-mediated inhibition of TPA-induced H2O2 formation was also comparable in both cell types. These results suggest that HL-60 cells can become a useful in vitro system to screen rapidly for chemopreventive agents and to study their properties.
PubMed ID: 7750119
MeSH Terms: Adult; Antineoplastic Agents/pharmacology*; Comparative Study; Dimethyl Sulfoxide; Female; Humans; Hydrogen Peroxide/metabolism*; Leukemia, Promyelocytic, Acute/drug therapy; Leukemia, Promyelocytic, Acute/metabolism*; Leukocytes/drug effects; Leukocytes/metabolism; Research Support, U.S. Gov't, P.H.S.; Tetradecanoylphorbol Acetate; Tumor Cells, Cultured