Title: Dynamic reprogramming of transcription factors to and from the subtelomere.
Authors: Mak, H Craig; Pillus, Lorraine; Ideker, Trey
Published In Genome Res, (2009 Jun)
Abstract: Transcription factors are most commonly thought of as proteins that regulate expression of specific genes, independently of the order of those genes along the chromosome. By screening genome-wide chromatin immunoprecipitation (ChIP) profiles in yeast, we find that more than 10% of DNA-binding transcription factors concentrate at the subtelomeric regions near to chromosome ends. None of the proteins identified were previously implicated in regulation at telomeres, yet genomic and proteomic studies reveal that a subset of factors show many interactions with established telomere binding complexes. For many factors, the subtelomeric binding pattern is dynamic and undergoes flux toward or away from the telomere as physiological conditions shift. We find that subtelomeric binding is dependent on environmental conditions and correlates with the induction of gene expression in response to stress. Taken together, these results underscore the importance of genome structure in understanding the regulatory dynamics of transcriptional networks.
PubMed ID: 19372386
MeSH Terms: Carbon/metabolism; Chromatin Immunoprecipitation; Cluster Analysis; Computational Biology/methods; DNA-Binding Proteins/classification; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Gene Expression Profiling; Heterochromatin/metabolism; Models, Biological; Protein Binding; Saccharomyces cerevisiae Proteins/classification; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism*; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism*; Telomere/metabolism*; Time Factors; Transcription Factors/classification; Transcription Factors/genetics; Transcription Factors/metabolism*