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Title: Purification of cytochromes P-448 from beta-naphthoflavone-treated rainbow trout.

Authors: Williams, D E; Buhler, D R

Published In Biochim Biophys Acta, (1982 Aug 27)

Abstract: Rainbow trout were treated with beta-naphthoflavone and the hepatic microsomal cytochrome P-450 solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Chromatography on tryptamine-Sepharose 4B gave a single cytochrome P-450 peak which was further resolved into three components by elution from DEAE-Sepharose. The two main peaks were then chromatographed on hydroxyapatite and a total of four fractions obtained. Two of these fractions had similar properties and significantly metabolized [14C]benzo[a]pyrene in a reconstituted system containing rat cytochrome P-450 reductase. This activity was inhibited by alpha-naphthoflavone but not by metyrapone of SKF-525A. Purified cytochromes P-448 from 3-methylcholanthrene-treated rat had similar spectral properties and activity towards [14C]benzo[a]pyrene suggesting similarities between these forms.

PubMed ID: 7126636 Exiting the NIEHS site

MeSH Terms: Animals; Benzoflavones/pharmacology*; Chemical Phenomena; Chemistry; Cytochrome P-450 CYP1A2; Cytochrome P-450 Enzyme System/isolation & purification; Cytochromes/isolation & purification*; Flavonoids/pharmacology*; Microsomes, Liver/drug effects; Microsomes, Liver/enzymology*; Rats; Solubility; Trout; beta-Naphthoflavone

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