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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Cytotoxicity of bacterial-derived toxins to immortal lung epithelial and macrophage cells.

Authors: Peterson, Dianne E; Collier, Jayne M; Katterman, Matthew E; Turner, Rachael A; Riley, Mark R

Published In Appl Biochem Biotechnol, (2010 Mar)

Abstract: Health risks associated with inhalation and deposition of biological materials have been a topic of great concern due to highly publicized cases of inhalation anthrax, of new regulations on the release of particulate matter, and to increased concerns on the hazards of indoor air pollution. Here, we present an evaluation of the sensitivity of two immortal cell lines (A549, human lung carcinoma epithelia) and NR8383 (rat alveolar macrophages) to a variety of bacterial-derived inhalation hazards and simulants including etoposide, gliotoxin, streptolysin O, and warfarin. The cell response is evaluated through quantification of changes in mitochondrial succinate dehydrogenase activity, release of lactate dehydrogenase, initiation of apoptosis, and through changes in morphology as determined by visible light microscopy and scanning electron microscopy. These cells display dose-response relations to each toxin, except for triton which has a step change response. The first observable responses of the epithelial cells to these compounds are changes in metabolism for one toxin (warfarin) and alterations in membrane permeability for another (gliotoxin). The other four toxins display a similar time course in response as gauged by changes in metabolism and loss of membrane integrity. Macrophages are more sensitive to most toxins; however, they display a lower level of stability. This information can be used in the design of cell-based sensors responding to these and similar hazards.

PubMed ID: 19172232 Exiting the NIEHS site

MeSH Terms: Apoptosis/drug effects; Bacterial Toxins/toxicity*; Biomimetic Materials/toxicity; Cell Line; Cell Membrane/drug effects; Cell Membrane/metabolism; Epithelial Cells/cytology*; Epithelial Cells/drug effects*; Epithelial Cells/metabolism; Epithelial Cells/ultrastructure; Humans; Inhalation; Lung/cytology*; Macrophages/cytology*; Macrophages/drug effects*; Macrophages/metabolism; Macrophages/ultrastructure; Microscopy, Electron, Scanning; Time Factors

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