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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Induction of heme oxygenase-1 in normal and malignant B lymphocytes by 15-deoxy-Delta(12,14)-prostaglandin J(2) requires Nrf2.

Authors: Bancos, Simona; Baglole, Carolyn J; Rahman, Irfan; Phipps, Richard P

Published In Cell Immunol, (2010)

Abstract: Heme-oxygenase-1 (HO-1) is induced in response to oxidative stress and is believed to be a cytoprotective and anti-inflammatory enzyme. It is unknown whether normal or malignant human B-lineage cells express HO-1. 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) is an interesting electrophilic lipid mediator able to increase oxidative stress in B cells. Here, we tested normal and malignant human B-lineage cells for their ability to express HO-1 in response to 15d-PGJ(2), as well as the signaling pathways required for HO-1 expression. 15d-PGJ(2) potently induced HO-1 protein expression in normal and malignant B cells. Malignant B cells exhibited a greater induction of HO-1 protein compared to normal B lymphocytes. Using siRNA directed against the transcription factor Nrf2 and B cells isolated from Nrf2-deficient mice, we show that HO-1 induction by 15d-PGJ(2) is dependent on Nrf2. These results show that, compared to normal B lymphocytes, malignant B cells have a greater capacity to increase their HO-1 protein levels in response to 15d-PGJ(2). We speculate that the ability to highly express HO-1 by malignant B cells could confer a survival advantage.

PubMed ID: 20064636 Exiting the NIEHS site

MeSH Terms: Animals; B-Lymphocytes/immunology; B-Lymphocytes/metabolism*; Cells, Cultured; Gene Expression Regulation, Enzymologic; Heme Oxygenase-1/metabolism*; Humans; Leukemia, B-Cell/immunology; Leukemia, B-Cell/metabolism*; Mice; Mice, Inbred C57BL; Mice, Knockout; NF-E2-Related Factor 2/deficiency; NF-E2-Related Factor 2/genetics; NF-E2-Related Factor 2/metabolism*; Prostaglandin D2/analogs & derivatives*; Prostaglandin D2/immunology; RNA, Small Interfering/genetics; Transcription, Genetic

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