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Publication Detail

Title: Glutathione deficient C57BL/6J mice are not sensitized to ozone-induced lung injury.

Authors: Johansson, Elisabet; Wesselkamper, Scott C; Shertzer, Howard G; Leikauf, George D; Dalton, Timothy P; Chen, Ying

Published In Biochem Biophys Res Commun, (2010 May 28)

Abstract: In this study we examined the role of the antioxidant glutathione (GSH) in pulmonary susceptibility to ozone toxicity, utilizing GSH deficient C57BL/6J mice that lack the expression of glutamate-cysteine ligase modifier subunit (GCLM). Gclm(-/-) knockout mice had 70% GSH depletion in the lung. Gclm(+/+) wild-type and Gclm(-/-) mice were exposed to either 0.3 ppm ozone or filtered air for 48h. Ozone-induced lung hyperpermeability, as measured by total protein concentration in bronchoalveolar lavage fluid, was surprisingly lower in Gclm(-/-) mice than in wild-type mice. Lung hyperpermeability did not correlate with the degree of neutrophilia or with inflammatory gene expression. Pulmonary antioxidant response to ozone, assessed by increased mRNA levels of metallothionein 1 and 2, alpha-tocopherol transporter protein, and solute carrier family 23 member 2 (sodium-dependent vitamin C transporter) was greater in Gclm(-/-) mice than in Gclm(+/+) mice. These results suggest that compensatory augmentation of antioxidant defenses in Gclm(-/-) mice may confer increased resistance to ozone-induced lung injury.

PubMed ID: 20417186 Exiting the NIEHS site

MeSH Terms: Animals; Bronchoalveolar Lavage Fluid/cytology; Cell Differentiation; Glutamate-Cysteine Ligase/genetics; Glutathione/deficiency*; Glutathione/genetics; Lung Injury/chemically induced*; Lung Injury/genetics*; Lung/drug effects; Mice; Mice, Inbred C57BL; Mice, Knockout; Ozone/adverse effects*; Pneumonia/genetics; Protein Biosynthesis; RNA, Messenger/metabolism

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