Title: In vitro assessment of the inflammatory response of respiratory endothelial cells exposed to particulate matter.
Authors: Qu, Song; Liberda, Eric N; Qu, Qingshan; Chen, Lung-Chi
Published In J Toxicol Environ Health A, (2010)
Abstract: Particulate matter (PM) is a ubiquitous environmental pollutant that has been associated with increased risk of cardiopulmonary mortality and morbidity in urban communities. An increasing body of evidence suggests that inflammation induced by PM may play an important role in the development of cardiovascular diseases. However, airway epithelial cell lines, instead of vascular endothelial cells, are commonly used to investigate the effects of PM with respect to cardiovascular effects. Thus, the present study was conducted using primary human vascular endothelial cells (HMVEC-LB1), human white blood cells (WBC), and their cocultures to evaluate their inflammatory responses to various PM exposures. Enzyme-linked immunosorbent assay (ELISA) kits were used to measure the concentrations of interleukin (IL)-6, soluble intercellular adhesion marker (sICAM-1), and soluble vascular cell adhesion marker (sVCAM-1) in HMVEC-LB1, WBC, and their cocultures after exposure to size-fractionated PM. Pretreatment of cells with inhibitors was performed in order to examine pathways involved in PM-induced cellular responses. IL-6 levels increased significantly in HMVEC-LB1 cells exposed to PM in both a time- and concentration-dependent manner. However, particle exposure for up to 24 h failed to induce any detectable production of sICAM-1 or sVCAM-1 in HMVEC-LB1 cells. IL-6 production in response to size-fractioned PM exposures failed to show evidence of relative importance of particle sizes in their abilities to induce proinflammatory responses. Lastly, cocultures with WBC significantly enhanced particle induced IL-6 release in HMVEC-LB1 cells in a synergistic manner. The present study suggests that HMVEC-LB1 cells can be successfully used as an in vitro model to examine effects of PM exposure.
PubMed ID: 20574913
MeSH Terms: Air Pollutants/analysis; Air Pollutants/toxicity*; Coculture Techniques; Endothelial Cells/drug effects*; Endothelial Cells/pathology; Enzyme-Linked Immunosorbent Assay; Humans; Inflammation/blood; Inflammation/chemically induced*; Inflammation/pathology; Inhalation Exposure; Intercellular Adhesion Molecule-1/blood; Interleukin-6/blood; Leukocyte Count; Leukocytes; Particle Size; Particulate Matter/toxicity*; Respiratory System/drug effects*; Respiratory System/pathology; Risk Assessment; Time Factors; Vascular Cell Adhesion Molecule-1/blood