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Title: Malondialdehyde-deoxyguanosine adduct formation in workers of pathology wards: the role of air formaldehyde exposure.

Authors: Bono, Roberto; Romanazzi, Valeria; Munnia, Armelle; Piro, Sara; Allione, Alessandra; Ricceri, Fulvio; Guarrera, Simonetta; Pignata, Cristina; Matullo, Giuseppe; Wang, Poguang; Giese, Roger W; Peluso, Marco

Published In Chem Res Toxicol, (2010 Aug 16)

Abstract: Formaldehyde is an ubiquitous pollutant to which humans are exposed. Pathologists can experience high formaldehyde exposure levels. Formaldehyde-among other properties-induce oxidative stress and free radicals, which react with DNA and lipids, leading to oxidative damage and lipid peroxidation, respectively. We measured the levels of air-formaldehyde exposure in a group of Italian pathologists and controls. We analyzed the effect of formaldehyde exposure on leukocyte malondialdehyde-deoxyguanosine adducts (M(1)-dG), a biomarker of oxidative stress and lipid peroxidation. We studied the relationship between air-formaldehyde and M(1)-dG adducts. Air-formaldehyde levels were measured by personal air samplers. M(1)-dG adducts were analyzed by a (32)P-postlabeling assay. Reduction room pathologists were significantly exposed to air-formaldehyde with respect to controls and to the pathologists working in other laboratory areas (p < 0.001). A significant difference for M(1)-dG adducts between exposed pathologists and controls was found (p = 0.045). The effect becomes stronger when the evaluation of air-formaldehyde exposure was based on personal samplers (p = 0.018). Increased M(1)dG adduct levels were only found in individuals exposed to air-formaldehyde concentrations higher than 66 microg/m(3). When the exposed workers and controls were subgrouped according to smoking, M(1)-dG tended to increase in all of the subjects, but a significant association between M(1)-dG and air-formaldehyde was only found in nonsmokers (p = 0.009). Air-formaldehyde played a role positive but not significant (r = 0.355, p = 0.075, Pearson correlation) in the formation of M(1)-dG, only in nonsmokers. Working in the reduction rooms and exposure to air-formaldehyde concentrations higher than 66 microg/m(3) are associated with increased levels of M(1)-dG adducts.

PubMed ID: 20707408 Exiting the NIEHS site

MeSH Terms: Adult; Air Pollutants, Occupational/analysis; Air Pollutants, Occupational/toxicity*; Biomarkers/blood; Biomarkers/metabolism; DNA Adducts/biosynthesis*; DNA Adducts/blood; Deoxyguanosine/biosynthesis*; Deoxyguanosine/blood; Dose-Response Relationship, Drug; Environmental Exposure/analysis*; Female; Formaldehyde/analysis; Formaldehyde/toxicity*; Humans; Italy/epidemiology; Leukocytes/drug effects*; Leukocytes/pathology; Male; Malondialdehyde/blood; Malondialdehyde/metabolism*; Reproducibility of Results; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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