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Title: Anticancer and anti-inflammatory effects of cysteine metabolites of the green tea polyphenol, (-)-epigallocatechin-3-gallate.

Authors: Lambert, Joshua D; Sang, Shengmin; Hong, Jungil; Yang, Chung S

Published In J Agric Food Chem, (2010 Sep 22)

Abstract: (-)-Epigallocatechin-3-gallate (EGCG) has been shown to have cancer preventive activity in vitro and in vivo. We have previously shown that EGCG can undergo conjugation to cysteine to form 2'-cysteinyl-EGCG and 2''-cysteinyl-EGCG. Studies of thiol-conjugated metabolites of methamphetamine indicate that such metabolites are not detoxified but retain biological activity. Here, we examined the growth inhibitory, pro-oxidant, and anti-inflammatory activities of the cysteine metabolites of EGCG. Both compounds dose-dependently inhibited the growth of colon cancer and intestinal cell lines. Both metabolites prevented aberrant arachidonic acid release and nitric oxide production by lipopolysaccharide-stimulated RAW264.7 cells. Under cell culture conditions, 2''-cysteinyl-EGCG produced H2O2 at a faster rate than EGCG. The results of the present study show that cysteine conjugates of EGCG retain the growth inhibitory, anti-inflammatory, and pro-oxidant activities of EGCG in vitro and may play a role in disease prevention in vivo. These results remain to be confirmed in vivo.

PubMed ID: 20718469 Exiting the NIEHS site

MeSH Terms: Animals; Anti-Inflammatory Agents, Non-Steroidal/chemistry; Anti-Inflammatory Agents, Non-Steroidal/metabolism; Anti-Inflammatory Agents, Non-Steroidal/pharmacology*; Antineoplastic Agents, Phytogenic/chemistry; Antineoplastic Agents, Phytogenic/metabolism; Antineoplastic Agents, Phytogenic/pharmacology*; Catechin/analogs & derivatives*; Catechin/chemistry; Catechin/metabolism; Catechin/pharmacology; Cell Line, Transformed; Cell Line, Tumor; Cell Proliferation/drug effects; Cysteine/analogs & derivatives*; Cysteine/metabolism*; Cysteine/pharmacology; Humans; Mice; Rats; Tea/chemistry*

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