Title: A microfabricated deformability-based flow cytometer with application to malaria.
Authors: Bow, Hansen; Pivkin, Igor V; Diez-Silva, Monica; Goldfless, Stephen J; Dao, Ming; Niles, Jacquin C; Suresh, Subra; Han, Jongyoon
Published In Lab Chip, (2011 Mar 21)
Abstract: Malaria resulting from Plasmodium falciparum infection is a major cause of human suffering and mortality. Red blood cell (RBC) deformability plays a major role in the pathogenesis of malaria. Here we introduce an automated microfabricated "deformability cytometer" that measures dynamic mechanical responses of 10(3) to 10(4) individual RBCs in a cell population. Fluorescence measurements of each RBC are simultaneously acquired, resulting in a population-based correlation between biochemical properties, such as cell surface markers, and dynamic mechanical deformability. This device is especially applicable to heterogeneous cell populations. We demonstrate its ability to mechanically characterize a small number of P. falciparum-infected (ring stage) RBCs in a large population of uninfected RBCs. Furthermore, we are able to infer quantitative mechanical properties of individual RBCs from the observed dynamic behavior through a dissipative particle dynamics (DPD) model. These methods collectively provide a systematic approach to characterize the biomechanical properties of cells in a high-throughput manner.
PubMed ID: 21293801
MeSH Terms: Erythrocyte Deformability; Erythrocytes/parasitology*; Erythrocytes/physiology; Flow Cytometry/instrumentation; Flow Cytometry/methods*; Humans; Malaria, Falciparum/parasitology; Microtechnology; Plasmodium falciparum/growth & development*; Pressure