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Title: Direct analysis of methylated trivalent arsenicals in mouse liver by hydride generation-cryotrapping-atomic absorption spectrometry.

Authors: Currier, Jenna M; Svoboda, Milan; de Moraes, Diogo P; Matousek, Tomás; Dĕdina, Jirí; Stýblo, Miroslav

Published In Chem Res Toxicol, (2011 Apr 18)

Abstract: Growing evidence suggest that the methylated trivalent metabolites of inorganic arsenic (iAs), methylarsonite (MAs(III)) and dimethylarsinite (DMAs(III)), contribute to adverse effects of iAs exposure. However, the lack of suitable methods has hindered the quantitative analysis of MAs(III) and DMAs(III) in complex biological matrices. Here, we show that hydride generation-cryotrapping-atomic absorption spectrometry can quantify both MAs(III) and DMAs(III) in livers of mice exposed to iAs. No sample extraction is required, thus limiting MAs(III) or DMAs(III) oxidation prior to analysis. The limits of detection are below 6 ng As/g of tissue, making this method suitable even for studies examining low exposures to iAs.

PubMed ID: 21361335 Exiting the NIEHS site

MeSH Terms: Animals; Arsenic/chemistry; Arsenic/metabolism*; Arsenic/toxicity; Cacodylic Acid/analogs & derivatives*; Cacodylic Acid/analysis; Cacodylic Acid/chemistry; Liver/chemistry*; Liver/metabolism; Methylation; Mice; Oxidation-Reduction; Spectrophotometry, Atomic/methods*; Water Pollutants, Chemical/chemistry; Water Pollutants, Chemical/metabolism*; Water Pollutants, Chemical/toxicity

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