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Title: Generation of a Slc39a8 hypomorph mouse: markedly decreased ZIP8 Zn²⁺/(HCO₃⁻)₂ transporter expression.

Authors: Wang, Bin; He, Lei; Dong, Hongbin; Dalton, Timothy P; Nebert, Daniel W

Published In Biochem Biophys Res Commun, (2011 Jul 01)

Abstract: Previously this laboratory has identified the mouse Slc39a8 gene encoding the ZIP8 transporter, important in cadmium uptake. ZIP8 functions endogenously as a electroneutral Zn(2+)/(HCO(3)(-))(2) symporter, moving both ions into the cell. The overall physiological importance of ZIP8 remains unclear. Herein we describe generation of a mouse line carrying the Slc39a8(neo) allele, containing the Frt-flanked neomycin-resistance (neo) mini-cassette in intron 3 and loxP sites in introns 3 and 6. Cre recombinase functions correctly in Escherichia coli and in adeno-Cre-infected mouse fetal fibroblasts, but does not function in the intact mouse for reasons not clear. Slc39a8(neo) is a hypomorphic allele, because Slc39a8(neo/neo) homozygotes exhibit dramatically decreased ZIP8 expression in embryo, fetus, and visceral yolk sac - in comparison to their littermate wild-type controls. This ZIP8 hypomorph will be instrumental in studying developmental and in utero physiological functions of the ZIP8 transporter.

PubMed ID: 21658371 Exiting the NIEHS site

MeSH Terms: Animals; Base Sequence; Cation Transport Proteins/deficiency*; Cation Transport Proteins/genetics*; Cells, Cultured; Embryo, Mammalian/metabolism; Female; Fibroblasts/metabolism; Gene Expression Regulation; Gene Expression/drug effects; Introns; Mice; Mice, Knockout; Molecular Sequence Data; Neomycin/pharmacology; Placenta; Pregnancy; Yolk Sac/metabolism

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