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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Generation of stable reporter breast cancer cell lines for the identification of ER subtype selective ligands.

Authors: Shanle, Erin K; Hawse, John R; Xu, Wei

Published In Biochem Pharmacol, (2011 Dec 15)

Abstract: Estrogen signaling is mediated by two estrogen receptors (ERs), ERα and ERβ, which have unique roles in the regulation of breast cancer cell proliferation. ERα induces proliferation in response to estrogen and ERβ inhibits proliferation in breast cancer cells, suggesting that ERβ selective ligands may be beneficial for promoting the anti-proliferative action of ERβ. Subtype selective ligands can be identified using transcriptional assays, but cell lines in which ERα or ERβ are independently expressed are required. Of the available reporter cell lines, none have been generated in breast cancer cells to identify subtype selective ligands. Here we describe the generation of two isogenic breast cancer cell lines, Hs578T-ERαLuc and Hs578T-ERβLuc, with stable integration of an estrogen responsive luciferase reporter gene. Hs578T-ERαLuc and Hs578T-ERβLuc cell lines are highly sensitive to estrogenic chemicals and ER subtype selective ligands, providing a tool to characterize the transcriptional potency and subtype selectivity of estrogenic ligands in the context of breast cancer cells. In addition to measuring reporter activity, ERβ target gene expression and growth inhibitory effects of ERβ selective ligands can be determined as biological endpoints. The finding that activation of ERβ by estrogen or ERβ selective natural phytoestrogens inhibits the growth of Hs578T-ERβ cells implies therapeutic potential for ERβ selective ligands in breast cancer cells that express ERβ.

PubMed ID: 21924251 Exiting the NIEHS site

MeSH Terms: Antineoplastic Agents/therapeutic use*; Apigenin/therapeutic use*; Breast Neoplasms/drug therapy*; Breast Neoplasms/metabolism; Breast Neoplasms/pathology; Cell Line, Tumor; Doxorubicin; Estrogen Receptor alpha/genetics; Estrogen Receptor alpha/metabolism*; Estrogen Receptor beta/genetics; Estrogen Receptor beta/metabolism*; Female; Flavanones/pharmacology; Gene Expression Regulation, Neoplastic; Genes, Reporter; Humans; Ligands; Luciferases/metabolism

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