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Title: Genomic analysis of the phenylacetyl-CoA pathway in Burkholderia xenovorans LB400.

Authors: Patrauchan, Marianna A; Parnell, J Jacob; McLeod, Michael P; Florizone, Christine; Tiedje, James M; Eltis, Lindsay D

Published In Arch Microbiol, (2011 Sep)

Abstract: The phenylacetyl-CoA (Paa) catabolic pathway and genome-wide gene expression responses to phenylacetate catabolism were studied in the polychlorinated biphenyl (PCB)-degrading strain Burkholderia xenovorans LB400. Microarray and RT-qPCR analyses identified three non-contiguous chromosomal clusters of genes that are predicted to encode a complete Paa pathway that were induced up to 40-fold during growth of LB400 on phenylacetate: paaGHIJKR, paaANEBDF, and paaC. Comparison of the available genome sequences revealed that this organization is unique to Burkholderiaceae. Parallel proteomic studies identified 7 of the 14 predicted Paa proteins, most of which were detected only in phenylacetate-grown cells, but not in benzoate- or succinate-grown cells. Finally, the transcriptomic and proteomic analyses revealed the induction of at least 7 predicted catabolic pathways of aromatic compounds and some aromatic plant products (phenols, mandelate, biphenyl, C(1) compounds, mevalonate, opine, and isoquinoline), as well as an oxidative stress response and a large group of transporters. Most of these genes were not induced during growth on benzoate or biphenyl, suggesting that phenylacetate or a metabolite may act as a signal that triggers multiple physiological processes. Identifying the components of the Paa pathway is important since the pathway appears to contribute to virulence of Burkholderia pathogens.

PubMed ID: 21519854 Exiting the NIEHS site

MeSH Terms: Acetyl Coenzyme A/metabolism*; Burkholderia/genetics*; Burkholderia/growth & development; Burkholderia/metabolism; Gene Expression; Genes, Bacterial; Genome, Bacterial; Oxidative Stress/genetics; Polychlorinated Biphenyls/metabolism; Proteomics; Transcriptome

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