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Publication Detail

Title: Multiplexed, ligation-dependent probe amplification for rapid and inexpensive HLA-DQB1 allelotyping.

Authors: Akers, N K; Curry, J D; Smith, M T; Bracci, P M; Skibola, C F

Published In Tissue Antigens, (2011 Oct)

Abstract: Many effective options exist to accurately type DNA for human leukocyte antigen (HLA) alleles. However, most of the existing methods are excessively costly in terms of overall monetary costs, DNA requirements, and proprietary software. We present a novel assay capable of resolving heterozygous HLA-DQB1 allelotypes at two digits, with even greater specificity for the HLA-DQB1*06 allele family, by using the multiplexed ligation-dependent probe amplification technology. This assay provides more specific allele data than genome-wide analysis and is more affordable than sequencing, making it a useful intermediate for researchers seeking to accurately allelotype human DNA samples.

PubMed ID: 21762399 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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