Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.


The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: Novel antibody capture assay for paraffin-embedded tissue detects wide-ranging amyloid beta and paired helical filament-tau accumulation in cognitively normal older adults.

Authors: Postupna, Nadia; Rose, Shannon E; Bird, Thomas D; Gonzalez-Cuyar, Luis F; Sonnen, Joshua A; Larson, Eric B; Keene, C Dirk; Montine, Thomas J

Published In Brain Pathol, (2012 Jul)

Abstract: Quantifying antigens in formalin-fixed tissue is challenging and limits investigation in population-based studies of brain aging. To address this major limitation, we have developed a new technique that we call "Histelide": immunohistochemistry (HIST-) and enzyme-linked immunosorbent assay (ELISA) (-EL-) performed on a glass slide (-IDE). We validated Histelide in sections of prefrontal cortex from 20 selected cases: 12 subjects with clinically and neuropathologically diagnosed Alzheimer's disease (AD), either autosomal dominant or late-onset forms, and 8 clinical and neuropathologic controls. AD cases had significantly increased amyloid beta (Aβ) peptide and paired helical filament- (PHF-) tau per area of neocortex that was proteinase K-sensitive, and significantly decreased amount of synaptophysin. We next investigated prefrontal cortex from 81 consecutive cases of high-cognitive performers from the Adult Changes in Thought (ACT) study, a population-based study of brain aging and incident dementia. As expected, latent AD was common in this group; however, our results quantified widely individually varying levels of Aβ peptides and PHF-tau among these high-cognitive performers. This novel approach obtains quantitative data from population-based studies, and our initial studies with high-cognitive performers provide important quantitative insights into latent AD that should help guide expectations from neuroimaging and prevention studies.

PubMed ID: 21999410 Exiting the NIEHS site

MeSH Terms: Aged; Aged, 80 and over; Aging; Alzheimer Disease/metabolism; Alzheimer Disease/pathology; Amyloid beta-Peptides/analysis*; Brain/metabolism; Brain/pathology; Enzyme-Linked Immunosorbent Assay/methods*; Female; Humans; Immunohistochemistry/methods*; Male; Middle Aged; Paraffin Embedding; tau Proteins/analysis*

to Top