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Title: Nontargeted nucleotide analysis based on benzoylhistamine labeling-MALDI-TOF/TOF-MS: discovery of putative 6-oxo-thymine in DNA.

Authors: Wang, Poguang; Fisher, David; Rao, Anjana; Giese, Roger W

Published In Anal Chem, (2012 Apr 17)

Abstract: A method for nontargeted analysis of modified nucleotides in DNA (and RNA) is reported based on labeling with benzoylhistamine (BH) followed by MALDI-MS. The method provides deoxynucleotide-specific detection, accurate measurement of molecular ions, high sensitivity, semiquantitation, and, to the extent studied to date, normalization of response within a factor of <3.

PubMed ID: 22409256 Exiting the NIEHS site

MeSH Terms: DNA/chemistry*; Female; Histamine/chemistry*; Humans; Limit of Detection; Nucleotides/analysis*; Placenta/chemistry; Pregnancy; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*; Staining and Labeling; Thymine/chemistry*

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