Title: Nontargeted nucleotide analysis based on benzoylhistamine labeling-MALDI-TOF/TOF-MS: discovery of putative 6-oxo-thymine in DNA.
Authors: Wang, Poguang; Fisher, David; Rao, Anjana; Giese, Roger W
Published In Anal Chem, (2012 Apr 17)
Abstract: A method for nontargeted analysis of modified nucleotides in DNA (and RNA) is reported based on labeling with benzoylhistamine (BH) followed by MALDI-MS. The method provides deoxynucleotide-specific detection, accurate measurement of molecular ions, high sensitivity, semiquantitation, and, to the extent studied to date, normalization of response within a factor of <3.
PubMed ID: 22409256
MeSH Terms: DNA/chemistry*; Female; Histamine/chemistry*; Humans; Limit of Detection; Nucleotides/analysis*; Placenta/chemistry; Pregnancy; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*; Staining and Labeling; Thymine/chemistry*