Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

National Institute of Environmental Health Sciences

Use this QR code to view the newest version of this document
Use this QR code to view the newest version of this document

Your Environment. Your Health.

Publication Detail

Title: Particle-induced artifacts in the MTT and LDH viability assays.

Authors: Holder, Amara L; Goth-Goldstein, Regine; Lucas, Donald; Koshland, Catherine P

Published In Chem Res Toxicol, (2012 Sep 17)

Abstract: In vitro testing is a common first step in assessing combustion-generated and engineered nanoparticle-related health hazards. Commercially available viability assays are frequently used to compare the toxicity of different particle types and to generate dose-response data. Nanoparticles, well-known for having large surface areas and chemically active surfaces, may interfere with viability assays, producing a false assessment of toxicity and making it difficult to compare toxicity data. The objective of this study is to measure the extent of particle interference in two common viability assays, the MTT reduction and the lactate dehydrogenase (LDH) release assays. Diesel particles, activated carbon, flame soot, oxidized flame soot, and titanium dioxide particles are assessed for interactions with the MTT and LDH assay under cell-free conditions. Diesel particles, at concentrations as low as 0.05 μg/mL, reduce MTT. Other particle types reduce MTT only at a concentration of 50 μg/mL and higher. The activated carbon, soot, and oxidized soot particles bind LDH to varying extents, reducing the concentration measured in the LDH assay. The interfering effects of the particles explain in part the different toxicities measured in human bronchial epithelial cells (16HBE14o). We conclude that valid particle toxicity assessments can only be assured after first performing controls to verify that the particles under investigation do not interfere with a specific assay at the expected concentrations.

PubMed ID: 22799765 Exiting the NIEHS site

MeSH Terms: Air Pollutants/chemistry*; Air Pollutants/toxicity; Artifacts; Biological Assay/standards; Cell Line; Cell Survival/drug effects; Humans; L-Lactate Dehydrogenase/metabolism*; Nanoparticles/chemistry; Nanoparticles/toxicity; Particulate Matter/chemistry*; Particulate Matter/toxicity; Sensitivity and Specificity; Soot/chemistry; Soot/toxicity; Tetrazolium Salts/chemistry*; Tetrazolium Salts/metabolism; Thiazoles/chemistry*; Thiazoles/metabolism; Titanium/chemistry; Titanium/toxicity

Back
to Top