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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: O-hydroxylamine-coupled alkaline gel electrophoresis assay for the detection and measurement of DNA single-strand breaks.

Authors: Luke, April M; Nakamura, Jun

Published In Methods Mol Biol, (2012)

Abstract: The ability to detect and measure DNA single-strand breaks has been the aim of numerous assays developed to assess genotoxicity. These methods often rely on alkaline conditions to denature DNA. However, alkaline treatment of DNA also introduces artifactual SSBs through the cleavage of alkali-labile sites resulting in confounded data. Here, we describe a modified alkaline gel electrophoresis assay coupled with a neutral O-hydroxylamine to obtain the measurement of true SSB formation.

PubMed ID: 22941612 Exiting the NIEHS site

MeSH Terms: Cell Line; DNA Breaks, Single-Stranded*; DNA/analysis; DNA/chemistry*; Electrophoresis, Agar Gel/methods*; Hydroxylamine/chemistry*

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