Skip Navigation

Publication Detail

Title: A genome-wide association analysis of temozolomide response using lymphoblastoid cell lines shows a clinically relevant association with MGMT.

Authors: Brown, Chad C; Havener, Tammy M; Medina, Marisa W; Auman, J Todd; Mangravite, Lara M; Krauss, Ronald M; McLeod, Howard L; Motsinger-Reif, Alison A

Published In Pharmacogenet Genomics, (2012 Nov)

Abstract: Recently, lymphoblastoid cell lines (LCLs) have emerged as an innovative model system for mapping gene variants that predict the dose response to chemotherapy drugs.In the current study, this strategy was expanded to the in-vitro genome-wide association approach, using 516 LCLs derived from a White cohort to assess the cytotoxic response to temozolomide.Genome-wide association analysis using ∼2.1 million quality-controlled single-nucleotide polymorphisms (SNPs) identified a statistically significant association (P<10(-8)) with SNPs in the O(6)-methylguanine-DNA methyltransferase (MGMT) gene. We also show that the primary SNP in this region is significantly associated with the differential gene expression of MGMT (P<10(-26)) in LCLs and differential methylation in glioblastoma samples from The Cancer Genome Atlas.The previously documented clinical and functional relationships between MGMT and temozolomide response highlight the potential of well-powered genome-wide association studies of the LCL model system to identify meaningful genetic associations.

PubMed ID: 23047291 Exiting the NIEHS site

MeSH Terms: Antineoplastic Agents, Alkylating/pharmacology*; Cell Line, Tumor; DNA Methylation; DNA Modification Methylases/genetics*; DNA Repair Enzymes/genetics*; Dacarbazine/analogs & derivatives*; Dacarbazine/pharmacology; Gene Expression Regulation, Neoplastic; Genome, Human; Genome-Wide Association Study; Humans; Polymorphism, Single Nucleotide; Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy*; Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics*; Precursor Cell Lymphoblastic Leukemia-Lymphoma/pathology; Tumor Suppressor Proteins/genetics*

Back
to Top