Title: HPLC-based assays for enzymes of glutathione biosynthesis.
Authors: White, C C; Krejsa, C J; Eaton, D L; Kavanagh, T J
Published In Curr Protoc Toxicol, (2001)
Abstract: Glutamate cysteine ligase and glutathione synthase carry out the two-step synthesis of glutathione. The fluorescent thiol-reactive compound monobromobimane is used to derivatize reaction products in an HPLC-based assay with fluorescence detection. The assay described in this unit can be adapted for tissue homogenates or cultured cells.
PubMed ID: 23045057
MeSH Terms: Animals; Cells, Cultured; Chromatography, High Pressure Liquid/methods*; Glutamate-Cysteine Ligase/metabolism*; Glutathione Synthase/metabolism*; Glutathione/biosynthesis*; Reference Standards; Spectrometry, Fluorescence